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We examined the effects of temperature and photoperiod on the thermogenic characteristics of brown adipose tissue in plateau pikas.

测定了高原鼠兔在驯化2周后(分4种处理:23℃,16L:8D;23℃,8L:16D;5℃,16L:8D和5℃,8L:16D)褐色脂肪组织的蛋白含量、线粒体蛋白含量和细胞色素c氧化酶活性的变化。

Brown adipose tissue is a kind of thermogenic organ of nonshivering thermogenesis in small mammals. The key element in BAT heat production is the uncoupling protein, a unique protein located in the inner membrane of BAT mitochondria.

褐色脂肪组织是小哺乳动物非颤抖性产热的主要器官,该组织具有一个独特的非偶联呼吸机制,它是由细胞线粒体内膜上的解偶联蛋白所决定的。

One of them was identified as aldehyde dehydrogenase 2, and the others were both identified as mouse RIKEN cDNA 2900053E13, suggesting that the protein might be post translationally modified.

通过对差异蛋白的胶内酶解和高效液相色谱/离子阱质谱鉴定,发现其中一个蛋白点为乙醛脱氢酶2(aldehyde dehydrogenase 2),另外两个差异点鉴定结果为同一个蛋白RIKEN cDNA 2900053E13,提示该蛋白在正常和心肌缺血心肌的线粒体内修饰状态发生了变化。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制,酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力,酶蛋白亚基间的相互作用,酶蛋白的折叠以及内运动状态与酶活力的关系,酶蛋白发挥功能时的结构特性;运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性,热稳定性机制;确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位;确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构;确定了一个新型的染色质蛋白Cren7的溶液三维结构;正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

This suggested that AIF and Endo G translocate from mitochondrion to nucleus in OGD/R.

说明OGD/R过程中AIF和EndoG可能由线粒体转位到核。

RESULTS: Compared with Mitomap database, some new variations were found, among which np652 G insertion and np716 T-G transversion were found only in cancerous tissues.

结果 (1)与mitomap线粒体数据库比较后发现一些新的变异位点,其中np652 G插入和np716 T-G颠换仅在癌组织中。

Although only 246bp sequenced in domain I, transition,transversion and percentage of divergence are higher than domain III.

灰雁和鸿雁mtDNA控制区序列转换数远大于颠换数,表明家鹅线粒体控制区序列在种间、控制区区域间表现出不同的变异模式。

It is tentative investigated the transferring of mitochondria by microinjection. It is found that the key of the success of transferring of mitochondria by microinjection is not the microinjected from pollen grain trema, but the cultivated of the microinjected pollen grain.

对显微注射转移植物线粒体的技术进行了探索性研究,结果表明,该技术的难点不在通过花粉粒萌发孔进行mtDNA的显微注射,而在注射后花粉粒的培养。

The experiment was conducted to understand the influence of high-copper from Tribasic copper chloride (TBCC diet on mitochondrial complex of broiler hepatocyte.

采用玉米-豆粕型饲粮研究铜对肉鸡肝细胞线粒体呼吸链复合物活性的影响。

To explore effect of copper on MPTP in liver cell, performance, blood index, hepatic function, growth hormone and histopathological change, copper sulfate, copper methionine and tribasic copper chloride had been used in this experiment, level of copper was 11mg/kg in control group, the three levels of each copper sulfate, copper methionene and TBCC added to the basas diet were 110mg/kg, 220mg/kg and 330mg/kg copper in experimental groups.

为了全面了解肉鸡饲料中铜元素对其肝细胞线粒体膜通透性转变孔、生产性能、血常规、肝功能、生长激素以及组织病理学变化的影响,本试验选用:硫酸铜、蛋氨酸铜、三碱基氯化铜作为铜源,基础日粮对照组饲料铜含量为Feedstuff推荐的11mg/kg,三个试验高铜组分别为:110mg/kg、220mg/kg、330mg/kg.360羽1日龄AA商品代肉鸡随机分为12组,每组30羽。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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