线粒体

The results showed that the organization and gene order of the mitochondrial genomes of these bivalve species studied were different from each other. The size, organization, gene numbers, and gene order of mitochondrial genomes in bivalves at different taxa were different.

结果发现，双壳贝类线粒体的基因组结构、基因排列顺序均互不相同；不同目、科和属之间线粒体基因组的大小、基因排列方式以及基因种类也存在明显的差异，尤其是基因排列方式没有明显的规律。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

In addition, spermiogenesis includes chromatin condensation, migration of the cytoplasm and mitochondria to the caudal pole of the nucleus, formation of a moderate axial nuclear fossa containing the proximal centriole and part of the distal centriole and presence of a short cytoplasmic canal separating mitochondria from the flagellum.

另外，精子发生过程中还包括染色质浓缩，细胞质和线粒体向细胞核的尾端迁移，在核的后端中轴位置上形成中等大小的核后凹，近端中心粒和远端中心粒的一部分嵌在核后凹之内，短的胞质内陷管将线粒体与鞭毛分隔开。

The ultramicrostructure in atrial myocytes displayed glycogen aggregation, karyopycnosis, sarcoplasmic reticulum distention and chondriosome swelling at 24 hours after rapid pacing.

经过24 h的快速起搏，心房肌细胞出现糖原聚集、核固缩、空泡样变以及肌浆网扩张、线粒体肿胀、部分线粒体嵴轻度溶解等超微结构的改变。

Moreover, chondriosome hypertrophy and vacuolar degeneration were found. The damage lessened in SVitC and LVitC groups. Both myofilaments and sarcomeres in IVIG and IVIG+VitC groups were almost normal, and the chondriosome was normal.

6心肌的电镜检测：对照组心肌肌丝排列紊乱，肌节断裂严重，线粒体肥大，并出现空泡变性；大、小剂量VitC组病变比对照组有所减轻；IVIG组和IVIG+VitC组肌丝排列紊乱明显减轻，肌节断裂比对照组减少，线粒体基本正常。

The long-term opening of mitochondria permeability transition pore results in the release of cytochrome C, apoptosis inducing factors, Ca2+ and other apoptosis factors into cytoplasm, which can destruct the whole cell structure and function by activating the main member of apoptosis protein family——caspase, or destroying the intranuclear chromoplasm independently, or affecting other protein relying on Ca2+, and finally lead to the formation of apoptotic body.

线粒体是真核生物能量和代谢的中心，也是细胞凋亡信号传导途径中起关键作用的细胞器，越来越多的研究表明，在凋亡信号的刺激下，线粒体发生膨胀，其外膜膜电位降低，外膜破裂后释放出膜间隙的诸多凋亡效应因子，在细胞凋亡中起着主开关的作用。

Changes of lung ultrastructures: The results of electron microscope showed that in group 1, there were swelling mitochondria was arranged in disorder, less matrix, and hyalomere appeared, thin double-deck membrane in 50% samples: Interalveolar septum stroma was with edema in 60% samples; Polymorphonuclear leukocyte gathered in vessel or emigrated out of vessel in 50% samples; In group 2, double-deck membranes of mitochondria were integral, densely matrix showed micro-granule shape in 90% samples; Pinocytosis in epithelial cells of type Ⅰ lung strengthened, being destroyed, and pinocytosis in endothelial cells strengthened in 10% samples; lnteralveolar septum stroma was with edema, no polymorphonuclear leukocyte gathered in vessel or emigrated out of vessel or corpuscule was empty in type Ⅱ alveolar epithelium in 20% samples.

肺组织超微结构改变观察：纯氧机械通气组50%标本线粒体肿胀、排列紊乱，基质变浅，出现透明区，双层膜变薄，60%标本肺泡隔间质水肿，50%标本多形核白细胞血管内聚集或游出血管外现象；34%氧浓度机械通气组90%线粒体双层膜较完整，基质致密呈细颗粒状；10%标本可观察到Ⅰ型肺上皮细胞有吞饮增强、破坏及内皮细胞吞饮增强；20%标本肺泡隔间质水肿；未见到多形核白细胞血管内聚集或游出血管外现象及Ⅱ型肺泡上皮板层小体排空。

Al stress badly destroyed the ultra-structure of root tip cells, the nucleus changed uneven, the numbers of vacuole and mitochondria increased but crista of mitochondria changed unclear and almost disappeared.

与对照相比，最明显的变化是细胞核结构变得不完整，同时空泡增多，线粒体数量增加，但线粒体脊比较模糊甚至消失。4。

In this study, we wanted to discuss the consequences and potential of the CO1 gene as a marker for phylogenetic reconstruction and DNA barcoding of this endemic cyprinid group.

本研究将验证线粒体CO1基因在东亚特有类群中进行DNA条形码研究的可行性，并且拟基于线粒体CO1基因序列探讨东亚特有鲤科鱼类的系统发育关系。

Although the monophyly of Cultrins was strong supported, the relationships of each groups in East Asian endemic Cyprinid taxa were not well resolved. The result indicated that the mitochondrial CO1 gene sequence provided less phylogenetic information to resolve the relationship of this East Aisan cyprinid group.

但基于线粒体CO1基因序列的系统发育分析，东亚特有类群内部各分类单元的相互关系依然没有得到很好的解决，结果表明线粒体CO1基因无法提供足够的系统发育信息来解决东亚特有鲤科鱼类内部类群的系统发育关系。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。