线粒体

MethodsOur study was to treat gastric ulcer by invigorating spleen and benefiting vital energy Chinese drugs through clinical observation and experimental research, and compared with mucosa protectant -gastropine and H2 receptor antagonist -famotidine about the mucosa mitochondrion 8-oxygen guanine DNA glycosylase, thymine glycol DNA glycosylase,3-methyl adenine DNA glycosylase, the effect of gastralgia, splenic asthenia and quality of ulcer recovery.

比较健中愈疡片、黏膜保护剂胃舒平以及H2受体拮抗剂法莫替丁对胃黏膜线粒体 8－氧鸟嘌呤DNA糖基化酶、胸腺嘧啶乙二醇DNA糖基化酶、3－甲基腺嘌呤DNA糖基化酶的影响，并进行健脾益气方药与黏膜保护剂胃舒平以及H2受体拮抗剂法莫替丁对胃溃疡愈合质量的比较和评价。

Oxamate can kill the tumor cell by restraining lactate dehydrogenase activity,obstructing the course of glucolysis and energy metabolism,and degrading the mitochondrial membrance potential.

结论草氨酸盐可以抑制人胰腺癌细胞panc-1的LDH活性，阻断其糖酵解，进而影响其能量代谢杀伤肿瘤；同时可能通过降低细胞线粒体膜电位而导致细胞凋亡。

The phylogenetic analysis of Gymnothorax was reported in this paper by being based on DNA sequences of mitochondrial Cytb gene.17 species of Cytb gene full-length sequences were obtained and their length of Cytb gene was 1140bp.All sequences were analysised by using biological software, the phylogenetic tree was constructed by using three kinds of common contribution phylogenetic tree methods and the phylogeny of 17 species of Gymnothorax was deduced.

本论文采用线粒体Cytb基因为分子标记，共获得裸胸鳝属鱼类17种Cytb基因序列全长为1140bp的部分序列，应用分子生物学软件对结果进行分析，使用3种常见的建树方法：NJ法、ME法和MP法重建系统发育树，对裸胸鳝属17种鱼类的系统关系进行研究，为探讨裸胸鳝属鱼类的分子进化，确定它们之间的关系提供分子生物学方面的证据。

Codon partition schemes were compared in phylogenetic analyses at subfamily level for Gyrinidae and at family level for Hydradephaga based on partial mitochondrial COⅠ gene sequences.

利用PAUP和MrBayes软件，对线粒体COⅠ基因序列3个密码子位置的数据模块分别进行了豉甲科和水生肉食亚目在亚科或科水平上的系统发育学分析，结果表明第二密码子数据模块获得了理想的分析结果。

However, the museum C. heterodon specimens were preserved in formalin. It is necessary to approach the methods of effectively extracting the genomic DNA from formalin-fixed C. heterodon, and the feasibility of using the extracted DNA in microsatellite-and mitochondrial analyses. In the present paper, the genomic DNA of ten formalin-fixed C.

然而，过去的铜鱼标本都保存在福尔马林溶液中，有必要探讨从福尔马林固定的铜鱼标本中有效提取基因组DNA的方法以及这些DNA用于微卫星和线粒体分析的可行性。

Heterodon specimens was successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205 bp of the control region of mtDNA from all of the ten specimens was successfully amplified.

本实验通过改进的酒精梯度浸泡法去除标本中的甲醛，然后用酚-氯仿抽提法成功地提取到了铜鱼标本的基因组DNA；设计引物后进行了线粒体和微卫星的PCR扩增，扩增产物经银染检测。

Heterodon specimens as successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205bp of the control region of mtDNA from all of the ten specimens was successfully amplified.

本实验通过改进的酒精梯度浸泡法去除标本中的甲醛，然后用酚－氯仿抽提法成功地提取到了铜鱼标本的基因组DNA；设计引物后进行了线粒体和微卫星的PCR扩增，扩增产物经银染检测。

The heterogenicity of mitochondrial DNA has impact on the system performance of the examination system mainly in identifying monoploid of examination samples and calculating the probability of individual recognition.

线粒体DNA异质性的出现，对检测体系的系统效能存在着影响，主要表现在对所检测样本的单倍型的认同及个体识别概率的计算上。

Mitochondrial DNA; non-coding region; coding region ; system performance ; heterogenicity

线粒体DNA；非编码区；编码区；系统效能；异质性

Finally, the phylogenetic relationship of taxa with complete mitochondrial genome from Hexapoda was reconstructed by concatenated amino-acid sequences of 12 proteins downloaded from GenBank.

通过结合克隆测序方法，降低了实验难度，提高了该策略的通用性和稳定性，从而建立起一套快速、精确地进行昆虫全线粒体基因组测序的实验体系。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。