线粒体

Obvious genetic divergence was observed between Nepal panda and those from Yunnan and Sichuan. Additionally, DNA sequence variations were also found in concolor gibbons.

此外，对来自云南和海南的黑冠长臂猿的线粒体DNA的序列分析证实了形态和地理分布上的亚种划分，并提示海南黑长臂猿可能是一个独立的种。

In this study, we analyzed 8 rare and endangered animals, including giant panda, Yunnan golden monkey, red panda, Asiatic black bear, rhesus monkey, slow lorries, Chinese pangolin and concolor gibbons from China and adjacent areas.

本文以我国珍稀动物大熊猫、滇金丝猴、小熊猫，亚州黑熊、间蜂猴、恒河猴、中国穿山甲、黑冠长臂猿为材料，采用蛋白和同工酶电泳以及线粒体DNA序列分析（包扩采用非损伤取样以毛发和陈旧皮张样品进行的分析）的方法研究珍稀动物的遗传多样性状况及其同物种濒危的关系以及根据遗传学数据确定保护的基本单元等。

Laser confocal microscopy was used to observe the locations of Mfn2 gene with and without PKA in cells.

激光共聚焦显微镜观察完整的和去除蛋白激酶A磷酸化位点的线粒体融合素2基因在细胞中的定位。

The nucleotide composition and the phylogenetic relationship among 14 species in Coreidae based on 412bp sequences of Cyt b gene were studied. The results showed: the average contents of T, C, A and G were 34.2 %、11.4 %、35.7 % and 18.7 %, respectively; The content of A+T (69.9 %) was obviously higher than that of G+C (30.1 %), and come up to 82.8% at the third position of codon.

以线粒体细胞色素b基因作为分子标记，首次对缘蝽科4亚科14种昆虫进行序列测定，获得Cytb基因412bp的序列片段，该片段中碱基T、C、A、G的平均含量分别为34.2%、11.4%、35.7 %和18.7 %，A+T平均含量为69.9 %，明显高于G+C含量（30.1 %）。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

Japonicus used in this experimentation is matemal inheritance.5 Sequence analysis of mitochondrial 16S rRNA and COI gene and molecular phylogeny of four species of sea cucumber500 bp 16S rRNA and 540 bp COI fragment were obtained by PCR method from mitochondrial DNA of four species of sea cucumber, Apostichopus japonicus, Holothuria fuscogilva, Cucumaria frondosa and Mensamaria intercedens. Variable sites, nucleotide diversity, average number of nucleotide differences and average number of nucleotide substitutions per site were calculated to analyze sequence difference, and phylogenetic trees were constructed by the NJ and ME method.

结果显示在来自这5组家系的100个后代中仅检测到母本的单倍型，刺参的线粒体遗传模式表现为典型的母系遗传。5 4种海参16S rRNA和COI基因片段序列比较及系统学研究采用PCR技术对山东荣成、长岛、俄罗斯和日本的刺参，黄乳海参，北大西洋瓜参，二色桌片参的16S rRNA和COI基因片段进行了扩增和测序，分别得到了长度约为500 bp和540 bp的片段。

In human microsomes and hepatocytes, RRW has inhibitory effects on CYP 3A 4 and CYP 2C 9 at high concentrations.

在高浓度条件下，于人体的线粒体和肝细胞中， RRW 对 CYP3A4以及 CYP2C9可以产生抑制作用。

The mitochondrial COⅠ gene sequences of 28 species of Cypraeidae including those of 11 species newly determined were used to reconstruct their phylogenetic trees using the neighbor-joining,maximum parsimony and Bayesian methods,aiming to clarify their phylogenetic relationships.

新测了分布于我国海南沿海的11种宝贝科软体动物的线粒体COⅠ基因序列，结合GenBank中17种该科动物的同源性序列片段，基于不同的数学模型，以邻接法、最大简约法和贝叶斯法构建了宝贝科分子系统树。

The levels of SOD and CAT significantly increased and MDA decreased in bifidobacterium group compared to radition damage group.Electronic microgragh of spine neurocyte and neurogliocyte showed that cytoplasm,cytoblast and plastiosome were significantly engorged ,and neurogliocyte apoptosised in earlier period. TLR2mRNA increased in C group compared to B group by RT-PCR. Cytochrome C in kidney increased in C group compared to B group, but bcl-2,NF-Кb decreased. Conclusions : Enteroclysm is better compared to intragastric administration.

双歧杆菌处理组肝组织中SOD和CAT水平明显高于放射损伤组(P.01)，同时MDA明显低于放射损伤组(P.01)；电镜结果显示：放射损伤组与双歧处理组的细胞浆、细胞核和线粒体均明显肿胀，双歧处理组同时具有早期凋亡现象；RT-PCR结果显示：双歧处理组TLR2mRNA的表达明显高于放射损伤组；免疫组化结果显示，双歧处理组Cytochrome C的表达明显优于放射损伤组，而NF-кBp65和bcl-2则相反。

The interaction of Bcl-2 family proteins has regulatory effect to the apoptosis mediated by cytomicrosome.

Bcl-2家族蛋白成员间的相互作用对线粒体介导的细胞凋亡起调控作用。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。