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The 12940 bp nucleotide sequence of the mitochondrial genome of a brachyura dacapod, Eriocheir japonica sinesis, was determined.

测定了短尾类十足目动物中华绒螯蟹的线粒体基因组12940 bp的核苷酸序列。

The 12940 bp nucleotide sequence of the mitochondrial genome of a brachyura dacapod, Eriocheir japonica sinesis,...

测定了短尾类十足目动物中华绒螯蟹的线粒体基因组12940 bp的核苷酸序列。

In this paper, the cloning, expression and phylogenetic analysis of AmphiSDHD and AmphiS27 from the gut cDNA library of amphioxus Branchiostoma becheri tisingtauense are carried out.AmphiSDHD, encoding the cytochrome b small subunit in mitochondrial succinate-ubiquinone oxidoreductase, was isolated from the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense.

本文从青岛文昌鱼(Branchiostoma belcheri tsingtaunese)肠cDNA文库中,分离得到克隆L095和094,经鉴定它们分别编码文昌鱼线粒体琥珀酸-泛醌氧化还原酶中细胞色素b的小亚基和核糖体蛋白S27(AmphiS27)。

Methods Rat calvaria osteoblasts were cultured in the presence of 10 μg/mL-500 μg/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope.

将成骨细胞分别在添加了不同浓度(10μg/mL~500μg/mL)的nHA或nAg-HA/TiO2的培养基中培养2、6、8、24、72及120h,通过透射电镜观察、细胞计数及MTT法检测细胞超微结构、细胞增殖及线粒体活性,并计算单位细胞呼吸率。

The phylogenetic relationships of Calycanthaceae were investigated by means of molecular systematics, which was based on three DNA sequences from three genomes, including chloroplast DNA trnL-F, nuclear 18S ribosomal DNA, and mitochondrial matR gene.

本文选取来自于三个基因组—核基因组、线粒体基因组和叶绿体基因组的3个DNA序列片段,对蜡梅科的系统发育进行重建,主要探讨了蜡梅科的系统位置和属间系统演化关系。

The purpose of this study was analysis and tracking the heredity stability of Alpaca through amplifying special fragment from mitochondrion genome. A pair of primer was designed according to those sequences of Camelidae animal submitted in GaneBank and a fragment of 1014 bp was cloned.

摘 要:本试验对GaneBank中登录的部分骆驼科动物线粒体12srRNA/tRNA-Val/16rRNA基因序列进行同源性比较,并借助DNAstar软件设计引物,扩增并进行序列分析,旨在揭示其遗传变异的规律。

Results The electron microscopical observation demonstrated that intracellular lanthanum could be found,most of which entered the hepatocytes and its mitochondria. The lanthanum granules were also found to deposite in the zone of tight junctions of bile canaliculi, indicating that the tight junctions had been damaged.

结果 镧示踪标记法显示镧颗粒进入了细胞及线粒体内;毛细胆管紧密连接区也出现了致密的镧沉积,并与肝细胞间隙的镧沉积物相连,表明毛细胆管紧密连接已开放。

The activities of succinate dehydrogenase in hepatocytes, alkaline phosphatase and thiamine pyrophosphatase on the wall of bile canaliculi became obviously less.Both the activities and localizations of TPPase had changed. Some TPPase from the damaged lysosome-like vesicles and Golgi saccules of hepatocytes discharged into the cytoplasm. TPPase reaction product in some bile canaliculi decreased.

酶细胞化学技术显示肝细胞线粒体琥珀酸脱氢酶和毛细胆管壁上的碱性磷酸酶活性明显减弱或消失,焦磷酸硫胺素酶的活性及定位也发生了改变, TPPase从损伤的高尔基复合体及溶酶体样小泡漏出进入胞浆,部分毛细胆管壁上的TPPase反应物减少。

The phylogenetic relationship of raptors and nighthawks were estimated by mitochondrial rRNA gene sequences in this study by comparing the partial basepair fragments of 12S rRNA and 16S rRNA genes among 5 species of Falcoiformes, 3 species of Strigiformes and 1 species of Caprimulgiformes sequenced in this experement, together with the homologous segments of mtDNA in other 17 species of birds downloaded from GenBank.

本文测定5种隼形目鸟类、3种鸮形目鸟类和1种夜鹰目鸟类的部分线粒体12S rRNA基因和16S rRNA基因,结合从GenBank下载的17种鸟类同源基因进行系统进化研究。

The present study eatablished genechip technique for Ceratitis capitata, C. rosa and C. cosyra, in which the mitochondrial cytochrome oxidase Ⅰ gene was used as molecular marker, and the DNA sequences of fruit flies (Diptera: Tephritidae) were used as target sequence. The genechip detection system for fruit flies was composed of detecting probes and quality-control probes for C.

本研究选择线粒体DNA细胞色素氧化酶Ⅰ基因为分子标记基因,以双翅目实蝇科昆虫DNA序列为目标,建立了我国进境植物检疫害虫地中海实蝇Ceratitis capitata、芒果小条实蝇C.cosyra和纳塔尔小条实蝇C.rosa等生物芯片检测方法。

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