线粒体

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

Electron microscopic morphometry of heart samples revealed typical diabetic alterations consisting that myofibril arranged disordly, mitochondril swelling, mitochondrial crista and breaking and solving to form the lysis of cistae; some glycogen and lipid droplet depositing in myofibril.

模型组心肌细胞肌原纤维排列紊乱、断裂、溶解，线粒体肿胀变性，线粒体嵴断裂、溶解，形成空白区，肌细胞中可见糖原脂滴沉着；与模型组比较，TXS组心肌细胞肌原纤维排列整齐，线粒体形态尚可，偶有线粒体外膜缺损。

Three major clusters of numt sequences ( cyt b numt、 ATP8+ATP6 numt and ND4+ tRNA-His+ tRNA-Ser numt) were particularity amplified and sequenced from 10 species within Galliformes and A.galericalata. Comparison numts sequences with homologous mitochondrial partial genes get the different evolutionary characteristics and evolutionary speed compared to mtDNA, then examine the age of insertion of numts using the sequence divergence of numt from the mtDNA. Finally, joined Coturnix japonica、 Coturnix chinensis、 Lophura leucomelana、 Lophura swinhoii and A.albifrons mitochondrial sequences in GenBank, we study the importance function to phylogenetic research of mitochondrial genes and homologous numts.The evolutionary rate of Numts is lower than their authentic sequences of mtDNA, cyt b and ND4 gene evolve faster than homologous numts 2.5 times; ATP8+ATP6 gene evolve faster than numts 18.9 times in Galliformes. Numts show less codon position bias and transition bias under not phylogenetic pressure.

根据2004年3月GenBank登录的红原鸡核基因组草图与其线粒体基因组比对发现红原鸡核基因组中存在3段较长的线粒体假基因，利用PCR方法扩增鸡形目10种鸟类和雁形目中鸳鸯核基因组中3段numt序列：cyt b numt、ATP8及下游ATP6 numt和ND4+tRNA-His+tRNA-Ser numt，测序后分别比较线粒体基因与核同源numt序列的进化特点和进化速率，确定numt序列插入核基因组的时间，以及结合GenBank已登录的日本鹌鹑、蓝胸鹑、黑鹇、蓝鹇和1个雁形目物种——白鹅雁(A.albifrons)相应序列探讨两种同源序列对于鸟类系统进化发育重建的重要作用。

Finding the frequency of occurrence of heterogenicity among people and making the method of examining the heterogenicity standardized by setting up the data of mitochondrial DNA will be of great sig...

通过建立mtDNA数据库，找出异质性在人群中的发生频率，并使检测异质性的方法标准化，对线粒体DNA的更有效检测具有重要意义；线粒体DNA编码区多态性位点在提高线粒体DNA系统效能中具有独到之处。

From using the same cervida for the Hydropotes intermis mitochondrial gene and its ratio of cytb,Detected 140 bp Variable sites,Insertion and deletion were not found.By comparison.

本文测定了麋鹿线粒体cytb基因全序列，并对麋鹿线粒体cytb基因全长1140bp进行了分析，利用同为偶蹄类鹿科的獐子线粒体cytb基因与其比对，检测到了140个变异位点，未发现插入和缺失。

Japonica sinensis to the putative ancestral pancrustacean (crustacean/insecta) gene order.

中华绒螯蟹线粒体基因组与推测的泛甲壳类原始的线粒体基因顺序相比，至少有12个基因发生了重排，造成线粒体基因排列顺序显著的变化。

Rearrangements have been occurred in at least twelve mitochondrial genes, which have drastically altered the gene order of the mitten crab E. japonica sinensis to the putative ancestral pancrustacean (crustacean/insecta) gene order.

中华绒螯蟹线粒体基因组与推测的泛甲壳类原始的线粒体基因顺序相比，至少有12个基因发生了重排，造成线粒体基因排列顺序显著的变化。

Methods: Fifteen cases were divided according to their clinical characteristics: 7 cases of MELAS, 4 cases of pure mitochondrial myopathy, and 4 cases of CPEO.

线粒体病患者15例，根据临床表现分为3组，线粒体脑肌病伴高乳酸血症、卒中样发作组7例，单纯型线粒体肌病组4例，慢性进行性眼外肌麻痹组4例。

A shot-gun DNA sequence strategy was employed,in which the mitochondrial genome library of Muntiacus reevesi has been constructed to obtain the complete mitochondrial genome sequence.

通过建立麂属动物小麂线粒体DNA文库、鸟枪法测序，获得了小麂线粒体基因组全序列并对其基因组成、蛋白质的编码序列、tRNA基因等结构作了详细分析，这也是国内有关哺乳动物线粒体基因组全序列的首次报道。

When the soil relative water content is 80%, the chloroplast and mitochondria cristae were regular. When the soil relative water content is 100%and 60%, the chloroplasts became roud in shape.There were bigger giqantic starch grains and fat droplets in the chloroplasts. Mitochondria cristae became swollen. When the soil relative water content is 40%and 20%, thylakoids became swollen and disorder in leaf cell, mitochondria cristae became obscured .

5在土壤相对含水量为80%时，叶绿体和线粒体能维持其结构和膜的完整性；在土壤含水量为100%和60%时，毛桃和石头扁桃叶片中的叶绿体变圆，出现大颗粒淀粉和脂类小滴，线粒体嵴膨胀；在土壤含水量为40%和20%时，叶绿体类囊体紊乱肿胀，线粒体嵴模糊或溶解。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。