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OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大,核/浆比大,核仁小,胞浆内细胞器少,可见少量内质网和小线粒体,整个细胞呈幼稚、未分化状态;ICAM-1免疫细胞化学检测阳性;损伤肝组织HE染色和ICAM-1免疫组化检测显示,肝损伤初期,Hering管周围OVCs增生并且ICAM-1阳性表达,随着肝损伤的加重,OVCs继续增生,并且,OVCs沿增生的肝纤维向肝小叶迁移,同时,ICAM-1阳性表达也继续增多,并逐渐向肝小叶弥散分布;透射电镜下观察到,肝损伤初期,OVCs在Hering管周围增生,肝纤维随之增生,增生的OVCs与肝纤维黏附生长。

The ultrastructure resultshowed that in model group blood intima was adema,broadening, evendiscontinuous and incomplete under blood vessel bast. In the blood themonocyte and the lymphocyte infiltrated to intension board. Manymacrophages could befound and there were massive all levels of dissolve theenzyme body and swallow slightly soak in its cytoblastema,The endosarc netand the ribose body was increased and outside the amnion the pseudopodappeared. Endothelial cell morphogenesis changed. It is obvious thatmitochondria was dropsy, cytoblastema was non-uniformity.

超微结构结果显示模型组血管内皮下内膜水肿、增宽,甚至间断不完整;血中单核细胞及淋巴细胞向内弹力板浸润,可见较多巨噬细胞,其胞浆内含大量各级溶酶体及吞噬小泡,内质网及核糖体增多,胞膜外出现伪足;内皮细胞形态发生改变,可见线粒体水肿,胞浆不均匀;中药治疗后,上述病理损害明显减轻。

The effect of all this is to strengthen the mitochondria, increase energy output and slow down the cell's aging process.

这些过程的结果加强了线粒体的功能,使其输出更多的能量并且延缓细胞衰老的过程。

Results: The concentrations of sIL-2R, IL-6 and ET in serum in asthma group were higher than that in control group (P.01), Manifestation of the lung tissue under electron microscope: the lung organism of the animal was infiltrated lamellarly by eosinophile granulocyte in the model set.

结果:模型组血浆中sIL-2R、IL-6和ET的含量与正常对照组比较明显增高(P.01),肺组织光镜和电镜下观察显示:模型组动物的肺组织可见嗜酸性粒细胞片状浸润,Ⅱ型细胞部分线粒体脊突消失、膨胀,板层小体减少,有较多排空现象。

Results:(1) All of the four concentrations (500ug/mL 1000ug/mh 2000ug/ml 4000ug/ml) of RTE could inhibit CEM cell growth characterized by apoptosis characterized by pyknosis, complete nucleic membrane, swollen mitochondria and extended ergastoplasm etc. The apoptosis body was shown at the same time.

结果:(1)500ug/ml、1000ug/ml、2000ug/ml、4000ug/ml的附子提取物均可诱导CEM细胞凋亡,在电镜下可观察,CEM细胞出现核固缩,核膜完整,线粒体肿胀,粗面滤质网扩张等改变,并能发现凋亡小体。

AIM: To study the effect of ERK inhibition on the mitochondrial potential change in dexamethasone-induced thymocyte apoptosis.

目的:研究阻断ERK途径对地塞米松诱导的胸腺细胞凋亡中线粒体膜电势的影响。

Those cells had large, round shape nuclei, with abundant euchromatin and distinct nucleoli, but little heterochromatin.

细胞浆体积小,有大量的粗面内质网和线粒体

In result of electron microscope, cells of contrast group have large nuclear, plenty of euchromatin and microvillus, and organells, such as mitochondrion, Endoplasmic reticulum and Golgi apparatus are less.

电镜观察发现未经LTA作用的对照组细胞核大,常染色质丰富,细胞浆中线粒体、内质网、高尔基体等细胞器少,微绒毛较丰富。

Distance values between two sequences were calculated using the molecular data of complete mitochondrial genomes derived from 20 Eutherian animals, then the distance matrix was obtained.

选取20种有胎盘哺乳动物,以它们的全线粒体基因组作为分子数据,计算两两之间的LZ复杂性距离,得出距离矩阵。

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这一模式非常关注商人的网络信用基础。

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