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Total genomic DNA was extracted from four marine fishes (Verasper variegates, Cleisthenes herzensteini, Turbot Scophthalmus maximus, red seabream Pagrosomus major) using a proteinase K and phenol-chloroform procedure. Long mtDNA fragments were amplified by long and accurate PCR using primers designed based on the mtDNA sequenses of Thunnus thynnus thynnus, flounder Paralichthys olivaceus and other related fishes.

用蛋白酶k酚氯抽提法从圆斑星鲽、高眼蝶、大菱鲆和真鲷4种海水鱼的肌肉组织中提取基因组DNA,根据金枪鱼、牙鲆及相关鱼类的线粒体DNA序列保守片断设计出扩增引物,扩增从16SrRNA到ND4之间约9400bp的mtDNA长片段。

Cleistogamous rice ; filament ; mitochondria ; spherosome

闭颖授粉水稻;花丝;线粒体;圆球体

The mitochondrial genomic sequence in snappers and cobia are similar to other vertebrate mitochondrial genomes with respect to gene order and genomic organization.

线粒体基因组的比对分析表明本文测定的mtDNA基因组的绝大部分区段与GenBank中现有的脊椎动物的序列有较高的同源性。

Therefore, it is very important to study the structure of coenzyme Q10 in the molecular level to make the mitochondrion disease pathology known.

因此,在分子水平上探寻辅酶Q_(10)的结构定会对揭示线粒体疾病病理起重要作用。

There was lots of organelle, such as rib somes, mitochondria in companion cell during the mature stage of SE. Also the plasmodesmata appeared in cell wall between SE and CC.

在筛管成熟期间,伴胞的细胞质较浓,内含辜富的核糖核蛋白体、线粒体等细胞器,与筛管相连的细胞壁上具有胞间连丝。

Mitochondrial fatty acid oxidation is decreased, and peroxisomal and microsomal oxidation is complementally upregulated to decrease fatty acid accumulation.

线粒体脂肪酸氧化作用减少,过氧化物和微粒体氧化作用代偿性上调以减少脂肪酸的累积。

The results showed as follow:①Chinese astringent persimmon were climacteric respiration fruit, the respiration climacteric stage occurred at speediness softening stage. Ethylene climacteric stage and respiration climacteric stage were almost occurred at one time.②The activities of EXP, PG, PE, CX all had increased, and EXP increased early with PG, PE, CX in turn in persimmon fruit during ripening and softening.③Concomitancy to fruit softening, ultrastructural changes in the mesocarp tissue were occurred at speediness softening stage, showed as disaggregation of middle layer of cell wall, appearance of fibre-like structures, disorganization of chloroplast and distortion of some mitochondria.

研究结果显示:①中国涩柿属呼吸跃变型果实,在其快速软化期出现呼吸跃变;乙烯跃变与呼吸跃变几乎同期出现;②在成熟软化过程中,EXP、PG、PE、CX的活性均有上升,EXP活性上升最早,随后依次是PG和PE、CX;PG活性上升幅度最大;③伴随果实软化,果肉组织超微结构在快速软化期中胶层解体、丝状体出现,叶绿体解体、部分线粒体变形;④1-MCP可延缓柿果实硬度下降,常温下延长保硬期5-7天。

Main ultrastructrual changes included cytoplasm degeneration, want of pinocytotic vesicle and phagosone, decreased organelles and granules, mitochondria swelling, nucleus condensation, etc.

主要包括:中性粒细胞质变性,吞噬泡欠缺,细胞器及细胞颗粒减少,线粒体肿胀,细胞核浓缩等。

Spermatozoa of Hemicentrotus pulcherrimus belong to the type of flagelloform, it has a coniform cell nucleus on its head, a small apical body on its forepart, four mitochondria, a couple of central granules, and a flagellum.

海胆精子与其他无脊椎动物的精子一样,属于鞭毛型,其头部包含一个圆锥形的细胞核,前端配有一个小的顶体,还包括4个线粒体、一对中心粒和一根鞭毛。

Methods The mutations in blood corpuscle mitochondrial DNA T2DM without consanguinity and controls were determined by polymerase chain reaction-restriction fragment length polymorphism.

采用聚合酶链反应-限制性片段长度多态性法对无血缘关系的糖尿病患者及正常对照个体的血细胞线粒体DNA进行突变分析。

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