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In DM group, decreasing of density of axoplasm, deposition of fat droplets, swelling and vacuolization of mitochondria and separation or disintegration in lamellar of myelin sheath of the sciatic nerve fiber could be observed, which could be partially improved by EA and KBK.

电镜形态学分析见造模两个月的糖尿病大鼠坐骨神经髓鞘板层有分离现象,线粒体嵴紊乱,有的线粒体嵴完全消失形成空泡线粒体,轴浆内有大量脂肪颗粒沉积;经电针和渴痹康治疗后能减轻这些病理改变。4。无论是电针治疗还是渴痹康治疗均无明显降血糖作用。

Feasibility study on application of DNA bar-coding technology in molecular identification of ScolytideaCO I gene of 15 bark beetle species collected from different regions and host is studied to discuss the feasibility of application of DNA bar-coding technology in Scolytidea molecular identification.

基于线粒体COⅠ基因的DNA条形码技术应用于小蠹科昆虫分子鉴定的可行性研究通过对采自不同地区、不同寄主的15个小蠹虫样本的线粒体COⅠ基因研究,对基于线粒体COⅠ基因的DNA条形码技术应用于小蠹科昆虫分子鉴定的可行性进行了探讨。

WLP-S-10 200mg/kg inhibited mitochondrial swelling,breakdown of membrane potential,depletion of mitochondrial ATP,and release of cytochrome C and AIF from mitochondria.

结果显示,WLP-S-10可以降低小鼠死亡率,降低血清ALT和AST;防止线粒体GSH及ATP的耗竭;减轻线粒体肿胀和膜电位崩溃;减少线粒体细胞色素C和AIF的释放。

The results showed that the volume density of mitochondriumand mitochondrial intermembrane crack increased according to ischemic time extension;The volume density ofmitochondrial crista decreased according to ischemic time extension;prominent defference occured after 45 min-utes of ischemia,The practice demonstrates that image analysis instrument has marked pre...

结果显示:线粒体线粒体外室的体密度随缺血时间延长而加大,线粒体嵴的体密度随缺血时间的延长而减少,缺血45min以后即和正常对照有显著性差别。实践证明,图像分析仪在定量超微结构的变化上,具有高度的精确性,故可早期发现病变。

The shape of chloroplast didn't change after seedling was treated by GA〓, but the number of lamllasomes increased, and starch grain enhanced. These changes were accord to that of the rate of photosynthesis. Mitochondria varied greatly, the structure of mitochondria could see vaguely and carinace almost deliquesce after 4days. Enrolucrum emerged until the seventh day after treatment and endomembrance of mitochondria recovered gradually, cristae could be seen. On the other hand, the endomembrance of mitochondria in CK (normal non-bolting) was obvious and the quantity of cristae in the mitochondria was much more than the GA〓 treatment. This showed that respiration might decrease after GA〓 treatment while reduced the depletion of photosynthesis products and enhanced bolting.

GA〓处理后抽苔过程中叶绿体形状未发生变化,但类囊体增多,淀粉粒增多,这种变化与光合速率的变化吻合;线粒体变化较大,处理后4天线粒体结构较模糊,嵴不清晰,7天时外膜形成,内膜也逐渐恢复,能见到嵴,而对照线粒体内膜结构明显,嵴清晰数量也多,说明GA〓处理后,呼吸作用降低,减少光和产物的消耗;细胞膜无破损,较光滑完整,说明对细胞膜没有伤害。

By 23rd March 2004, there have been 883 records about mtDNA of Chrysomelidae in the data bank of NCBI, but the record of cytb is 0. cytb is the protein-coding gene and known most clearly in mtDNA. Its often used to study Diptera but never to study the phylogeny of Chrysomelidae.

截至2004年3月23日,在NCBI数据库中,有关叶甲科线粒体基因记录共有883条,但线粒体细胞色素b基因为0记录。cytb基因是一种编码蛋白的基因,是目前了解的最清楚的线粒体基因之一,多用于研究双翅目昆虫,却未曾用于研究叶甲科的系统学问题。

Methods A total of 144 healthy Wistar rats were randomly divided into three groups, ie, sham operation group, obstructive jaundice and biliary tract recanalization and obstructive jaundice and biliary tract recanalization plus rhALR to respectively observe, at selected time points, the changes of the liver and mitochondrial function as well as morphologic changes of the mitochondriae by electroscope.

144只健康Wistar大鼠随机分为SHAM组、BDO??RBF组、BDO??RBF??rhALR组,分别检测其线粒体功能及肝功能的变化情况,电镜观察各组大鼠肝细胞线粒体的形态学改变。结果胆道梗阻后,大鼠线粒体功能及肝功能均出现明显损伤(P<0.05或P<0.01),BDO??

The study was carried out to investigate the influences of EGCG (--Epigallocatechin gallate on mitochondrial PTP opening and Ca2+ transportation.

线粒体膨胀及线粒体膜电位的研究发现,EGCG对Ca2+和H2O2导致的线粒体通透性改变有抑制其膨胀的作用。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤:(1)分别提取鱼类基因组DNA和线粒体DNA;(2)在鱼类线粒体DNA D-loop控制区设计简并引物:其上游引物MitD1-F有21个碱基:CAC CCY TRR CTC CCA AAG CYA;下游引物MitD1-R有23个碱基:GGT GCG GRK ACT TGC ATG TRT AA;(3)在引物的作用下进行PCR反应;(4)琼脂糖凝胶电泳;(5)对扩增的mtDNA D-loop基因片断进行测序。

Results showed that there were the morphological changes of mitochondria, such as vacuolization, degeneration and cristae swelling or disappearance, in HT-29 cells treated by C2-ceramide for 24 hours. Under the fluorescence microscope, mitochondria of apoptotic cells aggregated and emitted red fluorescence, however, mitochondria of normal cells emitted green fluorescence. The mitochondrial membrane potential began to decrease after 6 hours in HT-29 cells treated with C2-ceramide in time and dose dependent manner.

结果显示C2-神经酰胺作用HT-29细胞24小时,线粒体发生空泡化、变性、嵴肿胀或消失等形态学改变;此时荧光显微镜下可观察到线粒体聚集,发出红色荧光,而正常细胞线粒体发出绿色荧光。C2-神经酰胺作用细胞6小时,线粒体膜电位即开始降低,呈时间-剂量-效应关系。

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