线粒体

Three methods, which were SDH activity, specific fluorescent probe（Rh123） and mitochondrial 16S rDNA gene amplification, were used to detect sperm mitochondria of Eriocheir sinensis, and three tissues——muscle, branchia and testis, were investigated as control.

以中华绒螫蟹肌肉、鳃和精巢为对照，从线粒体标志酶SDH活性、线粒体特异性荧光探针（Rh123）以及线粒体16S rDNA PCR扩增3个途径，探讨了该蟹精子线粒体的存在状况。

Sertoli cells can provide a material basis and a favorable microenvironment for sperm. After X radiation, spermatogenic cells and mitochondrion dramatically swelling and lysis with a decrease of mitochondrial cristae, with the prolonging of time, the mitochondria damage get even worse, mitochondrial crista is disappeared.

Sertoli细胞在精子发生过程中可为精子发生提供物质基础和能力，为精子发生提供有利的微环境，对精子的生成有复杂的作用。X射线照射后即出现生精细胞和支持细胞线粒体明显肿胀，线粒体嵴减少，且随着时间延长，线粒体形态的损害进一步加重，线粒体数量减少，线粒体嵴消失。

To make it clear, using multilaser flow cytometry, molecular biologic technology and laser confocal microscope scan technology, we fixed eyes on mitochondrial permeability transition pores, mitochondrial transmembrane potential and caspases-3 (cysteinyl aspartate-specific proteinases) which are playing key roles of mitochondria. Meanwhile, the research also discussed the characteristics of Δψm of cells in cut specimenfrom esophageal carcinoma, adjacent tissue of esophageal carcinoma and the normal esophageal tissue.

为探讨食管癌细胞凋亡过程中线粒体所起的具体作用，本课题采用流式细胞检测、分子生物学和激光共聚焦扫描技术等手段，研究了食管癌细胞凋亡中，线粒体所起关键作用的环节—线粒体通透性转变孔(Mitochondrion Permeability Transition Pore，MPTP)、线粒体跨膜电位ΔΨm和线粒体释放反应中的Caspase-3(Cysteine protease-3)；同时研究了食管癌手术切除标本中癌灶、癌旁和切端三处组织的细胞线粒体跨膜电位的特性。

In the present paper, we determined the mitochondrial DNA sequence 539bp of the high variable I D-loop regions(mtDNA HVS-I) for 243 domestic fowls 19 were Wugu (or black-bone chicken and 8 non-wugu breeds/races and 38 red jungle fowls (Gallus. Gallus. spadiceus and Gallus. Gallus. jabouillei). We also sequenced 289bp mitochondrial DNA of Cyt-b for 5 red jungle fowls; D-loop region (mtDNA HVS-I)for 19 individuals Guinea fowl and 18 individuals mule chickens (Guinea fowl $ x Wugu chickens^ ) respectively; mtDNA HVS- II of D-loop region for 34 individuals of domestic fowls and red jungle fowls in 690bp and the MC1R code gene in 945 bp.

本文共测定了19个乌骨鸡品种及8个非乌骨鸡品种的243个家鸡和38个原鸡个体(属于Gallus.gallus.spadiceus和Gallus.gallus.jabouillee亚种)的线粒体D-环高变区(mtDNA HVS-Ⅰ)539bp的部分序列，另外还测定了5个红原鸡个体线粒体细胞色素b的289 bp序列、19个珍珠鸡个体、18个骡鸡个体的线粒体D环高变区序列、30个家鸡个体线粒体D环非高变区的690bp序列以及34个家鸡及原鸡个体的MC1R基因编码区的945bp。

This work is the first report about the complete mitochondrial genomes on A.melete and E. pyretorum.It adds two more Lepidopteran complete mitochondrial genome sequences,and has accumulated useful information for mitochondrial phylogenomics research of insect.

本研究首次对鳞翅目黑纹粉蝶A.melete和樟蚕E.pyretorum线粒体基因组进行了序列测定、拼接及线粒体基因组注释和分析，丰富了鳞翅目昆虫的线粒体基因组数据，为进一步开展昆虫线粒体谱系基因组学的研究提供重要参考。

The following five reviews published in this volume summarize recent discoveries in this area: DNA Replication and Transcription in Mammalian Mitochondria, Mitochondrial-Nuclear Communications, Translocation of Proteins into Mitochondria, The Machines that Divide and Fuse Mitochondria, and Why Do We Still Have a Maternally Inherited Mitochondrial DNA?

这一卷发表的以下五篇综述总结了这个领域的最新发现：哺乳动物线粒体中的DNA复制和转录，线粒体-细胞核通信，蛋白质到线粒体的迁移，分离和融合线粒体的机制及为什么我们仍然有一个母系遗传的线粒体DNA？

The Chinese Muntjac's mitochondrial genome, consisting of 16354 base pairs which encode genes for 13 proteins, 2 rRNAs, and 22 tRNAs, is similar to those mammals in both order and orientation.The sequence of rRNA gene ,some of the protein-coding regions and tRNAs are highly homologous in mammals. Differences existing in the length and sequence of the D-loop regions account for the variations in mammals mitochondrial genomes.

与其他哺乳动物线粒体基因组全序列的比较研究发现：全长为16 354bp的小麂线粒体基因组同样编码13种蛋白质、2种rRNA和22种tRNA，除了用于调控线粒体DNA复制和转录的D-Loop区以外，小麂线粒体基因组各基因长度、位置与其他哺乳动物相似，其编码蛋白质区域和rRNA基因与其他哺乳动物具有很高的同源性。

The mitochondrion and chloroplast are the semiautonomous organelles: the DNA of mitochondrion and chloroplast; the protein synthesis of mitochondrion and chloroplast; protein sorting and assembly of mitochondrion and chloroplast.

线粒体和叶绿体是半自主性细胞器：线粒体和叶绿体的DNA；线粒体和叶绿体的蛋白质合成；线粒体和叶绿体蛋白质的运送和装配。

To explore the feasibility of the mitochondrial DNA as transgenic vector, we translated the mtDNAs of the Antheraea pernyi, Diaphania pyloalis Walker and the fish to the silkworm. Through microscopic examination and mtDNA polymorphism analysis, we tested the maintenance of the foreign mtDNA in the silkworm and investigated the influence of the foreign mtDNA to the physiological behavior.

为了探索线粒体DNA作家蚕转基因载体的可行性，本实验将柞蚕、桑螟和鱼的线粒体DNA转入家蚕体内，通过着色镜检、线粒体DNA多态性分析检测了外源线粒体在家蚕细胞中的存留情况，并调查了外源线粒体对家蚕生长的影响。

To obtain more information of magnesium homeostasis in mitochondria, mTn-lacZ/LEU2 transposon library was transformed into mrs2 deletion mutant to screen for suppressor genes of MRS2. YMR166C, a member of mitochondrial carrier family, was identified as a suppressor gene of MRS2. Deletion of YMR166C gene can rescue the defects of mrs2 deletion mutant such as the decrease in mitochondrial magnesium concentration, Group II RNA splicing defect and growth defect on nonfermentable carbon source. For the first time we demonstrated YMR166C is involved in mitochondrial magnesium homeostasis.

为了增进对线粒体镁离子代谢调控基因的了解，利用酿酒酵母mTn-lacZ/LEU2转座子文库筛选MRS2的抑制基因，发现线粒体载体家族成员YMR166C基因的缺失可以挽救MRS2基因缺失的突变体的生长缺陷、II型内含子剪接缺陷，并可以调节线粒体镁离子浓度，首次发现YMR166C是线粒体镁代谢相关基因。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。