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We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

To avoid these ugly dashed lines, you could set the hover border style on your anchors to dotted but override this in IE, making them solid instead

为了避免这些难看的虚线，可以将锚上的鼠标停留边框样式设置为点线，但是在IE 中覆盖这个规则，让它们显示为实线

Methods:9 patients including 8 male and 1 female were included in this study.2 patients suffered from both side of ANFH,5 were righe side and 2 were left side.X-ray,CT and MRI were performed before the surgery.4 hips were classified as ARCO stage 1,7 hips were stage 2.All the patients were treated as follows:the area of NFH and cystis degeneration were detected and bouche decompression was done through X-ray with C-arm,and the necrotic bone was cleared for the pathologic diagnosis under the monitoring of arthroscopy.And then the non-cell based tissue engineering bone, which was the complex of autologous red bone marrow and osteoinductice absorbing material containing BMP,was transplanted into the body.

本组9例，男性8例，女性1例；双侧股骨头坏死2例，右单侧股骨头坏死5例，左单侧股骨头坏死2例；术前均行X线摄片、CT、MRI 检查，ARCO分期：Ⅰ期4髋，Ⅱ期7髋；C型臂X线透视下行股骨头坏死区和囊性变区定位、髓芯减压，关节镜监视下，刮除坏死骨送病理；植入由自体红骨髓与含BMP的骨诱导活性材料复合成的非细胞型组织工程骨，并用空心钛钉支撑股骨头软骨下骨质。

Another one Baoding Bougainville Foundry Machinery Co., Ltd. produced the Z148E-Seismic compaction modeling line, and with the support of the separate control of a sand handling system, six-position shape, the hourly capacity of 90 cosmetic, the size of sand box 860 * 680 * 300/300mm, two lines can produce 0.5 kg-100kg range of high-grade iron products.

另有一条保定维尔铸造机械有限公司出品的Z148E型微震压实造型线，及与之单独配套的自动控制砂处理系统一套，六工位造型，每小时可生产90整型，砂箱尺寸为860*680*300/300mm，两条线可生产0.5kg-100kg不等的高牌号铸铁产品。

Casting an otherwise Baoding Bougainville Machinery Co., Ltd. produced the Z148E-compaction microseismic modeling line, and with the support of the separate control of a sand handling system, six-position shape, the hourly capacity of 90 cosmetic, the size of sand box 860 * 680 * 300/300mm, two lines can produce 0.5 kg-100kg range of high-grade iron products.

另有一条保定维尔铸造机械有限公司出品的Z148E型微震压实造型线，及与之单独配套的自动控制砂处理系统一套，六工位造型，每小时可生产90整型，砂箱尺寸为860*680*300/300mm，两条线可生产0.5kg-100kg不等的高牌号铸铁产品。

In this article both stereo vision and line2laser scan sensor are integrated to CMM,according to this measuring system the bound2 aries or outlines of object are obtained by stereo vision and the ostensible data of object are achieved by line2laser scan sensor. Information fusion of multi2sensor can be utilized to segment 3D data、to abstract the geometry characteristic of the object、to construct the object modeling.

将立体视觉和线激光扫描传感器集成到三坐标测量机下，由立体视觉来获得物体的边界或轮廓信息、线激光扫描传感器获得物体表面的三维数据，利用多传感器的信息融合技术对三维数据进行分割，提取被测物体的几何特征，并在此基础上构建物体模型。

A mark indicating the boundary of such an interval.

刻度线，分度线指示这种间隔的''。

In the traditional leather line, sewing thread, canvas every industry application, but also in the nets with lines, cable lines, advertising light box cloth, sunshade RainXhigh-grade cloth, geotextile materials and canoe, military industry and other industries has been the promotion of .

在传统的皮革线、缝纫线、逢帆布行业不断得到应用，又在渔网用线、电缆用线、灯箱广告布、高档遮阳雨蘕布、土工材料及皮划艇、军事工业等行业得到了推广。

BPS ability of transverse diffusion at 30℃ was better than that at 18℃ and 25℃. There was 34.6% individuals could move 3.6 cm in 24 h at 30℃.

该线虫在30℃条件下的水平运动能力比在18℃和25℃的强，有34.6%的线虫用24h的时间运动到3.6cm处。

Study on the solution of brachistochrone and the effect of friction;2. Based on physics,it was proved concisely that the cycloid is both brachistochrone and isochronous pendulum.

介绍旋轮线的主要性质及其在日常生活与物理中的一些实例，并结合物理学知识对其最速降线与等时性进行简洁的证明。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。