纺锤

L sat with his knees drawn to his chest and his backside not touching the seat of his chair, which made it difficult to judge his body type, but from what Mello could see, L was a spindly man with alien-like fingers, unruly dark hair that seemed to stick out in all directions except straight up, and delicate, fine-boned wrists that belied the size of his large hands.

左旋坐到注意胸部和膝盖后方不触及自己的椅子上坐下，而难以判断其体质，但从梅洛能看见左旋是一个陌生男子纺锤形状的手指，漆黑的头发，好象棒粗野出全方位除外直线上升，而微妙，细保税手腕，他的大手挖大小。

Here we show that PRC1 recruits CLASP1 to the central spindle at early anaphase onset.

根据PRC1在中心纺锤体组成过程中的作用，我试图解析PRC1功能蛋白质群的分子构成及其作用机制。

During anaphase I of meiosis pairs of chromatids still connected at their centromere move to the spindle poles.

在减数分裂后期 I 中，由于纺锤丝的牵引，使成对的同源染色体各自发生分离，并分别向两极移动。

It has been proposed that extra centrosomes generate CIN by promoting multipolar anaphase, a highly abnormal division that produces three or more aneuploid daughter cells.

人们长期所持的一个观点是，额外的中心体通过诱导混乱的多极纺锤体组装和非对称的细胞分裂来促使染色体错误分离。

Condition optimization for its efficient fermentation was also carried out. The reaction and separation system with the addition of resin HD-8 and the isoeugenol/aquoeus biphasic system of the biotransformation of isoeugenol to vanillin by Bacillus fusiformis CGMCC1347 were set up. The isolation and purification of isoeugenol and vanillin in both systems were studied. The reuse of the immobilized cells in biphasic system was successfully applied.

SW-B9的基础上，对该菌株进行了生理生化特性鉴定以及16S rDNA序列分析，命名为纺锤芽孢杆菌CGMCC1347；继而对该菌株的发酵条件进行了研究，首次建立了纺锤芽孢杆菌CGMCC1347转化异丁香酚生成香草醛的树脂分离耦合体系和异丁香酚—水两相体系，同时对此二体系的底物和产物的分离提取进行了研究，成功实现了两相体系中固定化细胞的反复利用。

SW-B9 was selected for further study due to its highest vanillin production ability. According to physiological and biochemical characteristics of strain SW-B9 and the 16S rDNA gene sequence analysis, strain SW-B9 was identified as a kind of Bacillus fusiformis.

生理生化实验结合16S rDNA序列分析表明，该菌株为纺锤芽孢杆菌，命名为纺锤芽孢杆菌CGMCC1347，保藏于中国北京中关村中国微生物菌种保藏管理委员会普通微生物保藏中心，保存号CGMCC No.1347。

In order to obtain viable tetraploids, which remains a major challenge worldwide, research on spindle organization and chromosome behavior was carried out mainly in zhikong scallop, Chlamys farreri, a typical commercially important species which is being cultured in North China Sea. Immunofluorescent technique and laser scanning confocal microscopy has been developed for observation of both microtubule organization and chromosome segregation. Orcein squash method was also used for chromosome observation. A series of complicated configurations of the meiotic spindle and associated patterns of chromosome segregation, including tripolar and seperated bipolar segregation, were found in developing eggs following the inhibition of the first polar body.

为了攻克可存活四倍体人工培育技术的国际性研究难题，本研究选取了我国北方海区的典型经济贝类栉孔扇贝为主要研究对象，建立了一套免疫荧光染色和激光扫描共聚焦显微技术，结合使用染色体地衣红压片观察技术，对第一极体受抑制的受精卵的减数分裂纺锤体装配、染色体分离动态进行了研究，发现了一系列特殊的纺锤体装配及相应的染色体分离行为，包括三极分离、独立二极分离等分离类型，从亚细胞水平上阐明了四倍体形成的细胞学机理。

In prophase, it localized on the microtubule organizing-center. In metaphase, it co-localized with α-tublin completely on spindle. In anaphase, it co-localized with α-tublin in aster and mid-zone. In telophase, it co-localized with α-tublin in cytoplasmic bridge connecting the two daughter cells.

在分裂前期，hSMP-1蛋白位于微管组织中心区域；在分裂中期，hSMP-1蛋白与组成纺锤体的微管呈完全共定位；在分裂后期，hSMP-1蛋白与星体和纺锤体中区的微管呈共定位关系；在分裂末期，hSMP-1蛋白则与两个子细胞之间的胞质桥完全共定位。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

RESULTS: The rates of germinal vesicle breakdown were 98%, 22.2%, 40.1% and the rates of the first polar body (PB1) extrusion were 77.7%, 15.5%, 27.6% for M16, Hx and FA groups, respectively. The rates of GVBD and PB1 were all increased with the addition of FA compared with the Hx groups and comparison was significant (P.05). Hx and FA did not influence chromosome, but FA reversed the size of metaphase spindle elongated by Hx.

结果：M16培养液组、Hx模型组和FA处理组的GVBD率分别为98%、22.2%、40.1%，其PB1排出率分别为77.7%、15.5%、27.6%，各组间的差异均具有统计学意义(P.05)；次黄嘌呤和叶酸对染色体的形态没有影响，而次黄嘌呤使正常的纺锤体变长、变宽、面积变大，FA组较Hx模型组增大的纺锤体有所恢复，并接近于正常水平。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。