纯化的

The present invention also provides the affinity medium and the method for decontaminating urine prokinase and/or urokinase.

本发明还提供了新的用于纯化尿激酶原和／或尿激酶的亲和介质和方法。

The serum-free medium is obtained by: the purified human transferring was directly dissolved in the DMEM. Following detoxicating, BSA, cholesterol, insulin and hydrogen peroxidase were directly added in the DMEM, and diluted to a needed concentration.

所用无血清培养基血清替代物是将纯化人转铁蛋白直接溶于DMEM培养基中，再将去毒后的BSA、超声波粉碎后的胆固醇、胰岛素及过氧化氢酶直接加入DMEM培养基中稀释至所需浓度。

This suggested that the purified and dialyzed SjR47 fractional protein should have better immunogenicity and immunoreactivity, and share egg origin antigen.

实验结果表明，经纯化透析后的SjR47组分蛋白具有较高的免疫原性和免疫反应性，该蛋白分子属血吸虫卵源性组分抗原。

At the same time, the purified soil DNA was partially digested by enzyme Sau3AI, and the 2-6kb and 6-9kb fragments were recovered by dialyzing respectively.

纯化后的土壤DNA用限制性内切酶Sau3AI随机切割，以透析袋分别回收2-6kb和6-9kb的酶切片段，然后用T_4连接酶与上述脱磷载体连接。

The COL was nonspecific in agglutination for any type of human erythmeytes, and the hemagglutinating activities ware all at the same level.

纯化后的COL的最大紫外吸收峰在285nm，用SephadexG－200分子筛层析测得其分子量为4 252。

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。

The target gene was amplified,and PCR products were recovered,purified,and ligated with TA clone of pGEM-Teays.

扩增目的基因的，回收、纯化PCR产物，与pGEM-Teays载体的TA克隆连接。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后，在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素（hMG，0、0.1、0.2、0.5、1、2、5、10 IU/ml）单独或联合作用下进行体外培养，收集培养液，采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

Fetal nucleated cells in maternal peripheral blood:Frequency and relationship to gestetion age.

孕妇外周血中胎儿细胞的分离纯化及在产前基因诊断中的应用。

Results After passaged and purification, MSCs were confirmed to have the potential of differentiation.

结果 经多重纯化后，流式细胞仪鉴定获得的干细胞为CD44、Sca-1、CD31和CD45的MSCs，并具有分化潜能。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失