纯化的

The PILE protein was successfully purified and initially established a ELISA detection methods for the diagnosis of Legionnaires disease which used the type Ⅳ pilin protein PILE as the coating antigen.

成功纯化了Ⅳ型菌毛蛋白PILE，初步建立了一种以Ⅳ型菌毛蛋白PILE为包被抗原的诊断嗜肺军团菌病的ELISA检测方法。

Twenty-seven compounds were isolated from the methanol extract of the stems of Pinus massoniana by means of chromatographic methods, and their structures were elucidated as 8(14),15-Pimaradien-19-oic acid (1), Pimaric (2), Podocarpic acid (3), 12-Hydroxydehydroabietic acid (4), Dehydroabietic acid (5), 15-Hydroxydehydroabietic (6), 3β-Methoxyserrat-14-en-21-one (7), 3β-Hydroxy- serrat- 14-en-21-one (8), 3β-Methoxyserrat-14-en-21-one-30-al (9),(24R)-Stigmst- 4-ene-3-one (10),β-sitosterol (11),-3-Hydroxy-5-methoxy-stilbene(12), 4-Hydroxy-3- methoxycinnamaldehyde (13),-Ferulic acidtetracosyl ester (14), Alkylferulates (15), Pinocembin (16), Coniferaldehyde (17), Matairesinol (18),-Nortrachelogenin (19), Balanophonin (20), Cedrusin (21),-Pinoresinol (22), Tanegoside (23), 1,2-Bis-(4-hydroxy-3- methoxyphenyl)-propane-1,3-diol (24),(1S*, 2S*)-1-(4- Hydroxyphenyl) glyceri...

同时对马尾松枝条的甲醇提取物利用各种色谱技术进行了分离纯化，并利用现波谱技术鉴定了27个化合物的结构，分别为：8(14)，15-Pimaradien-19-oic acid (1)、海松酸(2)、罗汉松酸(3)、12-羟基脱氢松香酸(4)、脱氢松香酸(5)、15-羟基-脱氢松香酯(6)、3β-甲氧基-锯齿石松-14-烯-21-酮(7)、3β-Hydroxyserrat-14-en-21-one (8)、3β-Methoxyserrat-14-en-21– one-30-al (9)、(24R)-豆甾-4-烯-3-酮(10)、-3-羟基-5-甲氧基-二苯乙烯(12)、4-Hydroxy-3-methoxycinnamaldehyde (13)、-Ferulic acidtetracosyl ester (14)、Alkylferulates (15)、Pinocembin (16)、Coniferaldehyde (17)、罗汉松树脂酚(18)、 Balanophonin (20)、tanegoside (23)、1，2-Bis-(4-hydroxy- 3-methoxyphenyl)-propane- 1，3-diol (24)、(1S*，2S*)- 1-(4-hydroxyphenyl)glycerins (25)、(1R*，2R*)-1-(4-hydroxy- phenyl)gly-cerins (26)和赤松素(27)。

Some popular seawater phosphate δ^18 O analysis methods were reviewed. Then MAGIC-CePO4 coprecipitation-cation exchange resin separation method was choosed to preconcentrate, separate and purify the phosphate. Some basic experiment conditions were also discussed.

通过对国内外海水中磷酸盐氧同位素组成的测定方法进行查阅，选择改进后的MAGIC-CePO4沉淀－阳离子交换树脂法对海水中溶解态磷酸盐进行富集、分离与纯化，并对实验条件进行了探讨。

The preconcentration, separation and purification of phosphate was the foundation of seawater phosphate δ^18O analysis.

海水中溶解态磷酸盐的富集、分离与纯化研究是测定海水磷酸盐氧同位素组成的基础。

Their pure products of the six compounds were obtained by silica gel column chromatography and recrystallization in propanone solution.

通过硅胶柱色谱分离和重结晶对所得的六种化合物纯化后获得它们的纯化合物。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

Results: The results showed that both the wild type of recombinant MLCK and the ATP binding site mutagenesis of MLCK expressed in E.coli quantifiably with a large amount and exist as a soluble form.

结果：实验结果显示重组MLCK和MLCK/△ATP在大肠杆菌中以可溶的形式大量表达；经CaM-Sepharose 4B和Superose 6 HR纯化，SDS-PAGE鉴定得到单一的表达条带。

Polysaccharide was isolated and purified from Chinese medicinal herb Ramulus mori, and determination method for Ramulus mori polysaccharide was developed in this study. Carbon clearance test and dinitrylfluorobenzene-induced delayed type hypersensitivity test were used to evaluate effects of RMPS on immune responses of mice treated with dexamethasone.

采用水提醇沉法从中药材桑枝中分离纯化多糖，并建立桑枝多糖的含量测定方法；采用碳粒廓清法和二硝基氟苯诱导小鼠迟发型变态反应试验法，观察桑枝多糖对地塞米松所致免疫低下模型小鼠免疫功能的影响。

Healthy kunming mice mate to produce embryo. sacifice prognant mice at 14dpc, make preparation of fetal liver and born marrow. then proceed 40%-70% percoll gradient seperation to obtain nucleated erythroid cells. resuspend the purified erythroid cells in DMEM medium without serum, adjust cell concentration to 5×10〓.

正常健康昆明小鼠交配，待胚胎发育至14天时，处死怀孕母鼠，取胎鼠肝脏，用另一组成年小鼠制备骨髓；然后应用40％-70％Percoll梯度分离法分离胎肝和骨髓中的有核红细胞，纯化后的细胞用无血清DMEM培养基悬浮，调整细胞浓度为5×〓／ml。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。