纯化的

Isolation and purification and molecular characteristic of a myrosinase ,which was isolated and purified from the extraction by ammonium sulfate,DEAE-52 and Sephadex G-100 chromatography were studied.

以茎用芥菜为原料，采用磷酸缓冲液浸提、(NH4)2SO4盐析、DEAE-52和Sephadex G-100色谱对其中的芥子苷酶予以分离纯化，并就该酶的分子特性进行了研究。

In the second part of work, a basic peptide with mass weight of 7597 Da was isolated and purified from the Naja naja atra venom .

论文的另一部分工作通过对眼镜蛇毒进行分离纯化，获得了一个&极性很强&的组分，通过蛋白测序和分子量测定，组分确定为一个弱神经毒素，该毒素二级结构稳定，神经毒性很低。

College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,ChinaAbstract: To obtain the hybridoma cell line specific to 45W-4BX antigen of Taenia solium oncosphere,the recombinant plasmid pGEX-4BX containing TSO45W-4BX gene was highly expressed in Escherichia coli induced with IPTG.

为获得针对猪带绦虫六钩蚴45W-4BX抗原的杂交瘤细胞株，用IPTG对TSO45W-4BX的重组质粒pGEX-4BX进行了诱导表达，并采用Sepharose-4B层析技术对表达产物进行纯化，运用SDS-PAGE和Western-blot分别对其纯度和活性进行了检测。

Technological extrction process of f;avonoid compound with organical solvent was experimented .

摘要本文讨论了银杏叶中黄酮类化合物的提取、分离纯化并测定总黄酮的含量。

Wear particles in the osteolytic tissue can be obtained with strong base digestion and differential centrifugation, which is up to the demand of experimental study.

通过强碱消化及密度梯度离心提取、纯化假体周围骨溶解组织中磨损颗粒的方法可提供符合试验需要的金属颗粒。

Objective To study construction of vector to overexpress staphylokinase, expression in vitro and purification of the product.

目的研究高效表达葡激酶载体的构建及其体外表达与产物的纯化。

AIM: To construct,overexpress and purify a stable and active form of shortened human acidic fibroblast growth factor in Escherichia coli.

目的：稳定且具有活性的截断型人酸性成纤维细胞生长因子的构建、表达及纯化。

Coli. The antimicrobial activity of the recombinant fusion protein was evaluated in vitro, which exhibited high antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Pasteurella multocida, but low antimicrobial activity against Escherichia coil, and no antimicrobial activity against Salmonell choleraesuis.

对该重组蛋白进行纯化与体外抗菌活性的测定。结果表明，重组鸡AvBD6融合蛋白对多杀性巴氏杆菌、金黄色葡萄球菌、枯草芽胞杆菌有较高抗菌活性，对大肠杆菌的抗菌活性较弱，对猪霍乱沙门氏菌无抗菌活性。

Objective: This study was designed to establish the methods of isolation, culture and phenotypical analysis of dendritic cells from human peripheral blood.

目的 建立从人外周血分离、纯化、培养树突状细胞的方法，并研究细胞因子对树突状细胞体外增殖、分化成熟的影响。

Compared with pheophytin a and pheophytin b, the Soret bands of Cd-pheophytin a and Cd-pheophytin b red shifted by 19nm and 11nm, respectively.

6制备、分离和纯化得到镉叶绿素a和b的配合物，比较了它们与相应天然叶绿素的光谱性质。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失