纯化的

Optimum Extraction of Ursolic Acid in the Residues of Eriobotrya japonica by Orthogonal Test;2. After total saponins of Panax notoginseng were extracted,residues left was refined,separated,and purified.

通过对三七总皂苷提取后的药渣进行重新提取、分离与纯化得到了三七中另一有效成分———三七多糖，经含量测定可达50%，提高了三七的再开发利用价值。

Three strains of bacteria were isolated from haemolymph, hepatopancreas, ascites and muscle of the diseased Eriocheir sinensis caused by aeromonas. All of the three isolates were Gram-negative rods, motile by means of single polar flagella, and were facultatively anaerobic; Glucoce was catabolized with production of acid and gas; Oxidase was positive.

利用硫酸铵盐析、Sephadex G-100凝胶层析和DEAE-Cellulose离子交换柱层析等方法，从其胞外产物中分离纯化出了2种胞外蛋白酶，用SDS-PAGE电泳测得两种蛋白酶的分子量分别为39.6kD和20kD.39.6kD蛋白酶活性在50℃～60℃范围内热稳定性最好，最适pH为9，而20kD蛋白酶的最适温度为50℃，最适pH为8。

Effects of the amount of fumaric acid, temperature and reaction time on the reaction were investigated. Fumaropimaric acid was purified by potassium-salt method.

分别考察了富马酸加入量、反应温度及反应时间时富马松香制备的影响，并使用钾盐法对制备的富马松香进行纯化得到富马海松酸。

Methods: PNS was got by extracting panax notognseng with general method of alcohol circumfluence, purified with organic solvents, and separated with D101 macroreticular resin. The hematolysis rate and the immunocompetence of biology of PNS were determined by hemolysis test and experiment of splenocyte proliferation respectively.

乙醇回流法提取PNS粗提物，经有机溶剂除杂后，通过大孔树脂柱层析分离纯化；溶血性试验测定PNS作用后红细胞的溶血率；脾淋巴细胞增殖反应测定PNS的生物免疫活性。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法，从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干／祖细胞的单个核细胞(Mono-Nuclear Cell，MNC)，进行初步纯化及体外扩增后，组合使用Epo、IL-3、GM-CSF等细胞因子，将造血干／祖细胞向红系方向进行诱导培养。

Traditional method of screening homozygous transgenic plants is several generations self-pollenation, this is a time consuming work and low efficiency.

传统的纯化外源基因的方法主要是通过多代自交，该方法耗时长，效率低。

In the humus, the humin fraction is hardly to extract and purify, this limiting the study of its nature and structure.

其中胡敏素的提取和纯化很困难，从而限制了对其性质和结构的研究。

Electron microscopy study showed that immunotoxin rRTA:DS27 was routing along with a specific intracellular pathway.

结果：CM-Sephorose一步纯化到电泳纯的rRTA，电镜观察到rRTA：DS27遵循特异的胞内运输途径。

A fungus CTB430-1 with resistance to copper and zinc was isolated from soil at a copper and Zinc Tailing of Linxiang in Yueyang city, Hunan province. The fungus was identified by analyzing the morphology and measuring the ITS sequence. The effects of pH, initial concentration, co-ions on the growth of CTB430-1 and bioaccumulation of Cu2+ and Zn2+ were studied. The protein and glutathione in the cells were determined by using the coomassie brilliant blue method and iodometric method, respectively.

摘 要：从湖南省岳阳市临湘铜锌尾砂坝土壤内分离、纯化得到1株抗铜和锌的菌株CTB430-1，分析该菌株形态和测定菌株ITS基因序列鉴定菌种，研究不同pH值、金属离子初始浓度和金属离子共存对菌体生长量和富集重金属的影响；分别采用考马斯亮兰法和碘量法测定菌体内可溶性蛋白和还原性谷胱甘肽含量。

Methods Purification results were compared among the differential combination chromatography of anion and cation exchange, anion and cation exchange chromatography of both isocratic elution, and affinity chromatography.

阴、阳离子交换示差组合色谱法，以等度洗脱方法进行的阴、阳离子交换组合色谱，以及亲和色谱的纯化结果相对比。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失