纯化的

The crude protein was obtained by extracting the leaves of Blechnum orientale with PBS and precipitating with (NH4)2SO4. The crude protein was purified by Sephadex G－100 filtration after treatment through active carbon column.

乌毛蕨的叶片经PBS抽提和硫酸铵沉淀，得到的粗蛋白经活性碳柱处理后，在分子筛层析柱Sephadex G-100上纯化得到一种凝集素（Blechnum Orientale Lectin，简称BOL）。

Method8: The paPef, fOr the first time, studied the apoptosis or necrosis of K562cclls induced by bungarus multicinclus crude venom and several components frOmcrude venom by cation exchange chromatography\' through the quatitative analysis offlow cytometry as well as the qualitative analysis, such as inversed opticalmicroscopy, MTT method and fluorescent microscopy, and so on.

本卖验采用羧甲基纤维素C-52阳离子交换层析将银环蛇粗毒进行了若干毒素组分的分离纯化，并以流式细胞术定量分析为主，以倒置显微镜、MTT比色法、荧光显微镜等多种定性分析为辅，首次对银环蛇粗毒及其若干组分蛋白对人慢性粒细胞白血病K562肿瘤细胞的细胞杀伤作用和凋亡诱导情况进行了研究。

The immuneological functions of watery extract, sixty-five and ninety-five percent of ethanol extracts of Caesalpinia Sappan L. in mice were studied. Separation and identification of Components were primarily researched.

并利用水、65%和95%乙醇提取物进行了免疫药效学的动物实验研究，以及苏木中成分的分离纯化、鉴定等进行了初步探讨。

This text is the first one to report the preliminary test for the chemical components in the leaves of Carya cathayensis and C.

首次报道山核桃叶和美国山核桃叶化学成分预试验，有利于对其有效成分的提取、分离、纯化研究，为今后深入研究与开发利用该资源提供一定的研究基础。

Coli BL21(DE3) with the amount of 11.49% through thin layer chromatography scanning analysis. Cavia cobaya was immunized using the purified cytorrhyctes to produce the anti-serum. Through the determination of the optimum working conditions, the sensitivity test, the specificity test, repeatability test and sample simulation test, the indirect ELISA method was established, which is a broad-spectrum, rapid and specific to detect various of food-poisoning Vibrio simultaneously.

融合毒素蛋白包涵体经纯化，免疫豚鼠制备血清抗体，确定间接ELISA的最佳工作条件，并进行敏感性、特异性、重复性及模拟样品检测试验，建立了食物中毒性弧菌广谱、快速、特异的同步免疫学检测方法。

A protein, designated as cerebrin 30 (Mr. 30, 000), has been isolated and purified from human cerebrospinal fluids obtained from patients with schizophrenia disorders. The methods applied are C8 reversed-phase HPLC, SDS-PAGE in conjunction with HPEC. The N-terminal amino acid sequence of cerebrin 30 (〓-APEAQVSVQPLFXQ) was determined. Specific polyclonal antibody against cerebrin 30 was also prepared.

近来，国外研究人员应用C8反相HPLC、SDS-PAGE以及HPEC等方法从精神分裂症患者脑脊液中纯化了一种蛋白质，定名为Cerebrin 30（Mr.30，000），测得了其N-末端的十几个氨基酸序列，并制备了这一蛋白质的特异性多克隆抗体。

It can be used to purify the extract of Chaenomeles speciosa Nakai.30% and 50% ethanol showed better elution property.

DM-301型大孔吸附树脂表现出较好的综合性能，可以用于纯化资木瓜黄酮；用30%和50%的乙醇作为洗脱剂较好。

Four experiments were primarily studied the antimicrobial ability and activity of the antimicrobial peptides crude sample from young Chelydra serpentina's intestines,blood,liver,skin,moreover were studied the blood antimicrobial peptides' purification process and their related characteristic.

本文初步研究鳄龟肠道、血液、肝脏和皮肤组织粗提物的抗菌活性，在此基础上进一步研究了鳄龟血液抗菌肽的分离纯化过程及其相关性质。

The study on the extracting small experiment of Choleic acid, Glycocholic acid and Taurocholic acid.The extracting small experiments of Choleic acid, Glycocholic acid and taurocholic acid were studied by adopting deproteinizatio, depigmentation, extraction and column chromatographic fractionation.

提取胆酸、甘氨胆酸、牛磺胆酸的小试工艺研究采用脱蛋白、除色素、萃取、柱层析分离等方法开展了从牛羊混合胆汁中分离、提取和纯化胆酸、甘氨胆酸及牛磺胆酸三种有效成分的小试提取工艺研究。

Then the extract was distributed between dichloromethane and water, and the organic part was then subjected to HSCCC for the isolation of chromone constituents.

以芦荟全叶为原料，经过一系列的预处理手段，从脱色剂活性炭中获得芦荟色酮粗提物，再经过溶剂分配和富集后采用高速逆流色谱对其中的色酮成分进行分离纯化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失