纯化的

Pollen allergens from Carvota mitts and Caryota ochlandra were purified and characterized. The result will provide a basis for the diagnosis and treatment of allergy to the pollen allergens from Carvota mitis and Caryota ochlandra.

对长穗和短穗鱼尾葵花粉变应原进行了初步的分离、鉴定和纯化，为临床鱼尾葵花粉变态反应疾病的诊断和治疗奠定了基础。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot.

重组短穗鱼尾葵花粉profilin在大肠杆菌中高效的表达，进一步经Ni2＋亲和层析柱纯化后经Western-blot检测具有良好的免疫学活性。

Two of these protein bands, with a molecular weight of 12 000 Mr and 14 000 Mr, showed immunoreactivity with IgE in the sera from patients with allergy to pollen of Caryota ochlandra. For the pollen extract of Carvota mitis, 30 protein bands were detected in SDS-PAGE, and three of these protein bands, with a molecular weight of 12000, 14000, and 26000 Mr, showed immunoreactivity with IgE in the sera from patients with pollen allergy of Carvota mitis. The 14000 Mr protein was the major allergen. Result from the ion-exchange chromatography indicated that the two pollen allergens (12 000、14000 Mr) from Caryota ochlandra were eluted in the second peak. The major pollen allergen of Carvota mitis was eluted in the forth and fifth peak.

结果 长穗鱼尾葵花粉有30余条蛋白带，其中主要条带有18条，12000和14000 Mr为长穗鱼尾葵花粉特异性变应原；短穗鱼尾葵花粉有30余条蛋白带，其中主要条带有9条，26000、1200和14000 Mr为短穗鱼尾葵花粉特异性变应原，其中14000 Mr为主要变应原；通过离子交换层析方法纯化出长穗鱼尾葵花粉相对分子质量为12000和14000的变应原主要分布在Ⅱ峰中，短穗鱼尾葵花粉相对分子质量为14000阳的主要变应原分布在Ⅴ峰和Ⅵ峰中。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

Mold of 34 species were isolated from moldy books.Among them,3 species which were demonstrated to be the key mould causing book mold were identified morphologically and molecularly as Chaetomium globosum,Aspergillus terreus and Aspergillus fumigatus.

从霉变的书籍中分离纯化了34种霉菌，对其中的3种主要霉菌进行了形态学和分子生物学鉴定，分别鉴定为球毛壳菌种、土曲霉种、烟曲霉种。

Methods BALB/c mice were inoculated muscularly with HIV-2 gag recombinant DNA vaccine, or recombinant fowlpox virus, or both.

大量制备并纯化HIV-2 gag重组DNA疫苗和重组鸡痘病毒，以肌肉注射的方式免疫BALB／c小鼠，ELISA法检测小鼠血清HIV-2抗体，流式细胞仪测定CD4^＋、CD8^＋T淋巴细胞亚类数量，乳酸脱氢酶释放法检测脾CTL对HIV-2靶细胞的杀伤活性。

ConclusionIt has good effect to separate total alkaloids from embryo of Nelumbo nucifera gaertn seed by macroporous resin DA201 which can be used for a long time.

结论DA201大孔吸附树脂对莲心总碱的分离纯化效果良好，树脂使用寿命长，具有较好的工业化前景。

Min-1.ConclusionIt has good effect to separate total alkaloids from embryo of Nelumbo nucifera gaertn seed by macroporous resin DA201 which can be used for a long time. There is preferable industrialized foreground.

min-1。结论DA201大孔吸附树脂对莲心总碱的分离纯化效果良好，树脂使用寿命长，具有较好的工业化前景。

To detect the partial molecular characteristics of a novel fibrinolytic protease from the coelomic fluid of Nereis virens identified and purified to homogeneity.

目的：从沙蚕Nereis virens的体腔液中纯化、鉴定出一种新的纤溶酶，并对其进行分子表征。

Coli can overproduce 38 000 antigen of Mycobacterium tuberculosis. Inclusion bodies are easy to purify and can protect the recombinant antigen from protease, on the other hand, it has not biological activity unless the denatured protein is accurately folded.

构建的大肠杆菌重组体能高效表达结核分支杆菌38 000蛋白质抗原，包涵体的表达形式有利于蛋白质纯化和蛋白质稳定，蛋白质必须经过正确折叠才具有生物学活性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失