纯化的

The toxic compound was traced by lettuce germination test guided fractionation and HPLC purification, and was identified as 3β-formyl usolic acid on the basis of 1H and 13C NMR experiments. It is the first time to find this compound from the order of Myrtaceae

用高效液相色谱对桉树凋落叶中毒性物质进行分离、纯化，以小麦、绿豆、大速生菜为指示植物，对分离到的物质进行毒性跟踪，分离到一个毒性较强的组分，经1H氢谱和NMR鉴定为3–β甲酰基–乌索酸。

Methods The recombinant fowl pox virus transfer vector pSY681-gD-LacZ carrying gD gene of ILTV HNCG strain was transfected to chick embryo fibroblast infected with FPV-017 strain, then recombinant FPV was purified by blue plaque screening and identified by PCR and indirect IFA.

将含有ILTV河南长葛株gD基因的禽痘病毒转移载体转染禽痘病毒感染的鸡胚成纤维细胞，采用蓝色蚀斑筛选纯化重组禽痘病毒，并经PCR及间接免疫荧光试验进行鉴定。

To determinant the functional motif of S protein involved in the interaction with ACE2, seven truncated S proteins deleted from N or C terminal were obtained by E.

为了确定S蛋白与ACE2结合的功能区域，我们在大肠杆菌中表达了7个从N端截短或从C端截短的S蛋白，并用Ni柱纯化。

This means of affinity chromatography with gallinaceous ovomucoid has been proven to be a kind of economical and applicable method to purify and isolate WGA.

鸡卵黏蛋白亲和色谱法是一种经济适用的纯化麦胚凝集素的好方法。

Further investigation of the macroscale preparation and especial the purification of genistein is still in progress.

对于染料木素的中试规模制备，尤其是染料木素纯化工艺条件的优化进行了深入探索。

The factors affecting Aspergillus niger No.5.1 for β-glucosidase purification and enzyme properties have been investigated.

研究了来源于曲霉No.5.1的β-葡萄糖苷酶的纯化和酶学性质。

The expression vector pET32a-ITF was constructed and expressed in E.coli successfully, and the recombinant protein rITF and its antibody was also purified in this study. ITF expressed in small intestine goblet cell.

成功构建了表达载体pET32a-ITF，在大肠杆菌中表达并纯化获得纯度较高的rITF，并获得了生物活性较高的ITF抗体，ITF主要在肠道杯状细胞分泌表达。

AIM To improve the purification method of Human Chorionic Gonadotropin.

目的 ：提高人绒毛膜促性腺激素的活性回收率，简化 HCG的纯化步骤。

On the basis of guanidine thiocyanate method, an economical method of extracting and puring total RNA from Yunjie stigmas was established.

本文在常规异硫氰酸胍法的基础上，改进并确立了一种适合芸芥柱头总RNA的提取与纯化方法。

Total RNA extracted by improved guanidine thiocyanate method and treated with DNaseⅠwere found to be applicable to mRNA differential display technique.

提出了一种有效的芸芥总RNA提取和纯化方法改进异硫氰酸胍法提取的总RNA经DNaseⅠ处理后完全适用于DDRT-PCR分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。