纯化的

Methods We collected 43 microtia cases' peripheral blood and extract genome DNA. Design primers of three exons of the GSC, then sequence after PCR amplification and depuration sequencing.

采集43例小耳畸形患者的外周血提取基因组DNA，对GSC基因的三个外显子分别设计引物，经PCR扩增纯化后直接测序。

BACKGROUND: Muscle-derived stem cells have some problems in purification, amplification and directional differentiation during in vitro culture. Basic fibroblast growth factor as a membrane of bioactive factor has been paid great attention, due to its biological activity.

背景：肌源性干细胞在体外培养过程中存在纯化、扩增、定向分化等难题，碱性成纤维细胞生长因子作为生物活性因子的一员，因其生物活性的多效性而备受关注。

The results of experiment show the optimal extraction condition of Gardenia Yellow: refluxing and extracting three times with 50 percent ethanol in 55℃, water-bath and extracting 4 to 5 hours, Gardenia Yellow extract solution is separated by X-5 absorption resin, and then with 30 percent ethanol, getting Geniposide white crystal, the determining purity by HPLC come to 99.1% and can be used as standard, the part doffing by 70% ethanol can be concentrated to get more pure Gardenia Yellow pigment.

实验证明了栀子黄的最佳提取条件：50%的乙醇回流提取3次，55℃水浴加热，提取时间为4-5个小时。栀子黄提取液经X-5大孔吸附树脂分离，用30%乙醇溶液洗脱，纯化得到栀子苷白色晶体，HPLC检测纯度达99.1%，可做标准品使用。70%乙醇洗脱部分浓缩可得栀子黄产品，精制后产品纯度有显著提高。

The molecular weights of the 11 major bands were 82, 79, 60, 51, 46, 38, 32, 30, 28, 24 and 18kD, respectively. The results of Western blotting with the positive serum of anaphylactic patients showed that the 1st-4th instar larvae all had the specific allergens whose molecular weights were 82 and 79kD, respectively; only the 5th instar larvae all had the specific allergen of 30kD. We purified the protein of 30kD by ion exchange chromatography and gel isolation and identified it as ectoblast protein by MALDI-TOF-MS.

选用家蚕过敏患者阳性血清进行免疫印迹，1~4龄家蚕均显示出82和79kD的特异性变应原；但只有5龄家蚕的30kD蛋白为特异性变应原，通过离子交换层析和经切胶纯化出30kD蛋白，再经MALDI-TOF-MS鉴定该蛋白为外膜蛋白。

After fermentation and product purification, we got some purified fusion protein SOD-Thyα1. And the Enterokinase digestion of fusion protein was also studied.

经发酵和纯化得到了融合蛋白SOD-Thyα1的纯品，并对融合蛋白的肠激酶切割特性进行了初步研究。

In conclusion, the preparation method of rhIL-11 from fusion protein by using enterokinase is simple and feasible with good separation, which can meet industrial requirements.

利用肠激酶加工融合蛋白以制备rhIL-11的方法简单可行，分离纯化效果好，能够满足工业生产的需要。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

Objective:To study hydrolysis of vitamin E acetate catalyzed by cholesterol esterase in the reversed micelle systems.

研究了有机相脂肪酶催化合成共轭亚油酸β-谷甾醇酯的工艺条件，同时研究了甾醇酯的分离纯化方法。

In this paper, the techniques of fermentation , separation and purification of acarbose were studied.

本论文对阿卡波糖的发酵及分离纯化工艺进行了深入的研究。

The purification of protoplasts from cellular and subcellular debrids by floatable washing method with a modified centrifuge tube is described.

本文叙述了用改良离心管漂洗纯化原生质体中的细胞和亚细胞碎片的方法。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。