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Four further single mutants ofN546(N546G,N546D,N546K,N546Δ), three double mutants and a triple mutant were constructed,and toxins were purified by isoelectric precipitation and an AKTA purifier 100 system.When bioassayed against H.armigera using purified toxins,N546ALC_(501.67μg/mLshowed 1.78 times increased toxicity than wide-type Cry1AcLC_(50 2.98μg/mL,and mutant N546D,N546K and N546Δshowed a great loss in toxicity.

对N546进一步突变,获得了突变子N546G、N546D、N546K和删除突变N546Δ及3个双突变和1个三突变,利用等电点沉淀法和AKTApurifier 100系统纯化了毒素蛋白,生测发现N546ALC_(50为1.67μg/mL对棉铃虫毒力比野生型Cry1AcLC_(50为2.98μg/mL提高了1.78倍,而N546D、N546K和N546Δ的毒力则降低了80~90%。

This paper reviews biological characteristics of IgY, its extraction, purification, and application in immunodiagnosis and treatment.

本文就从IgY在生物学特性、提取、纯化,以及其在免疫诊断和免疫治疗方面的应用作一综述。

Methods Mitochondria were isolated from rat cerebral cortex and purified with a homogenizing and centrifuging program.

组织匀浆梯度离心法分离并纯化大鼠脑皮质线粒体,氧电极法鉴定线粒体氧化呼吸及其偶联活性;体外无细胞线粒体3H UTP和3H Leucine掺入法分别测定细胞器的转录与翻译活性。

The tall oil from waste fluid in papermaking,first purified by reduced pressure distillation , then some composite additives were added.

使用造纸废液中塔尔油,经减压精馏纯化,适当加入复合添加剂,制得优质醇酸树脂,从而制备出性能较好的工业底漆。

After isolating and purificating,the crystals were identical to authentic L-Tryptophan,in respects of melting point,optical activity and IR-spectra.

经分离纯化所得的L-色氨酸晶体,在熔点、旋光性和红外吸收光谱等方面与标准品完全一致。

Methods: The activity and purity of monocytes/macrophages isolated with MACS and traditional method were compared.

比较运用MACS法和传统方法分离、纯化妊娠早期外周血和蜕膜组织单核巨噬细胞的效果。

Extraction and purification of pumpkin polysaccharides were studied in the paper.

研究南瓜多糖的提取、分离与纯化。

The recombinant protein GFP-SA was purified through the Ni-NTA affinity chromatography, and then refolded.

用IPTG诱导重组蛋白的表达,用镍金属螯合层析柱进行纯化。

Inclusion bodies of expressed proteins were dissolved in denaturants and purified by NTA-Ni gel and refolded under suitable conditions.

用变性剂溶解包涵体蛋白,经Ni-NTA琼脂糖纯化后在适宜的条件下进行重折叠复性。

Motive:Extracting pure AchR from American Torpedo California electric organ, immuning Lewis rats actively, duplicating EAMG animal model.

中文摘要:目的:从美国加利福尼亚电鳐的电器官中提取纯化乙酰胆碱受体,主动免疫Lewis大鼠,复制实验性自身免疫性重症肌无力动物模型。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。