纯化的

Four further single mutants ofN546(N546G,N546D,N546K,N546Δ), three double mutants and a triple mutant were constructed,and toxins were purified by isoelectric precipitation and an AKTA purifier 100 system.When bioassayed against H.armigera using purified toxins,N546ALC_(501.67μg/mLshowed 1.78 times increased toxicity than wide-type Cry1AcLC_(50 2.98μg/mL,and mutant N546D,N546K and N546Δshowed a great loss in toxicity.

对N546进一步突变，获得了突变子N546G、N546D、N546K和删除突变N546Δ及3个双突变和1个三突变，利用等电点沉淀法和AKTApurifier 100系统纯化了毒素蛋白，生测发现N546ALC_(50为1.67μg/mL对棉铃虫毒力比野生型Cry1AcLC_(50为2.98μg/mL提高了1.78倍，而N546D、N546K和N546Δ的毒力则降低了80～90%。

This paper reviews biological characteristics of IgY, its extraction, purification, and application in immunodiagnosis and treatment.

本文就从IgY在生物学特性、提取、纯化，以及其在免疫诊断和免疫治疗方面的应用作一综述。

Methods Mitochondria were isolated from rat cerebral cortex and purified with a homogenizing and centrifuging program.

组织匀浆梯度离心法分离并纯化大鼠脑皮质线粒体，氧电极法鉴定线粒体氧化呼吸及其偶联活性；体外无细胞线粒体3H UTP和3H Leucine掺入法分别测定细胞器的转录与翻译活性。

The tall oil from waste fluid in papermaking,first purified by reduced pressure distillation , then some composite additives were added.

使用造纸废液中塔尔油，经减压精馏纯化，适当加入复合添加剂，制得优质醇酸树脂，从而制备出性能较好的工业底漆。

After isolating and purificating,the crystals were identical to authentic L-Tryptophan,in respects of melting point,optical activity and IR-spectra.

经分离纯化所得的L-色氨酸晶体，在熔点、旋光性和红外吸收光谱等方面与标准品完全一致。

Methods: The activity and purity of monocytes/macrophages isolated with MACS and traditional method were compared.

比较运用MACS法和传统方法分离、纯化妊娠早期外周血和蜕膜组织单核巨噬细胞的效果。

Extraction and purification of pumpkin polysaccharides were studied in the paper.

研究南瓜多糖的提取、分离与纯化。

The recombinant protein GFP-SA was purified through the Ni-NTA affinity chromatography, and then refolded.

用IPTG诱导重组蛋白的表达，用镍金属螯合层析柱进行纯化。

Inclusion bodies of expressed proteins were dissolved in denaturants and purified by NTA-Ni gel and refolded under suitable conditions.

用变性剂溶解包涵体蛋白，经Ni-NTA琼脂糖纯化后在适宜的条件下进行重折叠复性。

Motive:Extracting pure AchR from American Torpedo California electric organ, immuning Lewis rats actively, duplicating EAMG animal model.

中文摘要：目的：从美国加利福尼亚电鳐的电器官中提取纯化乙酰胆碱受体，主动免疫Lewis大鼠，复制实验性自身免疫性重症肌无力动物模型。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。