纯化的

The purified vWF was immobilized onto a coverslip. Cell rolling was induced in a parallel-plate flow chamber and observed by phase-contrast video microscope.

纯化的vWF固着在盖玻片上，在平行板液流室中进行细胞滚动研究，用相差电视显微镜观察。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

Objective: To optimize the isolation and purification of rat islets.

目的：探讨大鼠胰岛分离纯化的方案。

Designing and building machinery for commercial production took several more years. Finally, in 1986 (thirty-four years after Dr. Brohult's original discovery) the first purified shark liver oil containing a standardized dosage of alkylglycerols was marketed commercially.

设计和制造商用生产设备花了好几年时间，最终，在1986年第一个含标准剂量alkylglycerols、经过纯化的鲨鱼肝油才正式上市。

Liu KewuAbstract: Sucrase was isolated and purified from small intestine of pig and its properties had also been examined.

对猪小肠蔗糖酶(Sucrase，E.C.3.2.1.26)在分离纯化的基础上，对其性质进行了初步研究。

It was proved that the dipeptides could be synthesized through the two-liquid-phase enzyme membrane reaction with high yield and puri.

研究证明，由于反应和纯化的同步进行，二肽可以在双液相酶膜反应器中达到高纯度和高收率合成。

It indicated that this four methods can obtain pure plasmid DNA.

表明四种方法均能获取纯化的质粒DNA。

Result 4 mol/L sodium acetate solution, pH 13.0 and dying for 1 h were the optimal conditions of purification in gel slices.

结果 4 mol/L醋酸钠溶液、pH13.0、作用1 h为切胶纯化的最佳条件。

High performance rosin esters were produced by esterification reaction of pentaerythritol and toll oil rosin which was purified by reduced pressure distillation .

使用普通塔尔油松香和经过减压蒸馏纯化的精制塔尔油松香为原料，适当加入复合添加剂，通过酯化反应制得优质塔尔油松香改性树脂。

Methods BALB/c mice were immunized with purified sporozoites of E. acervulina . Hybridoma cell lines were set up by using hybridoma technique, and monoclonal antibodies were prepared.

使用纯化的堆型艾美球虫子孢子直接免疫BALB/c小鼠，采用杂交瘤技术建立细胞株，制备单克隆抗体。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失