纯化的

Mothod Using negative pressure moist liposuction and then injecting pure fat granule to the breast for 171 cases.

171例经过负压湿性吸脂后，注入纯化脂肪颗粒至乳房内，对其产生的并发症进行观察。

Recombinant wild type PrP purified from E. coli was used to form amyloid fibrils.

将 E。 coli 中纯化得到的重组蛋白质进行淀粉样蛋白质纤维之培养。

Total RNA was extracted from the head kidney of Siniperca chuatsi immunized with immersion then cDNA was synthesized by reverse transcription.

从浸泡免疫后的鳜头肾中提取总RNA逆转录合成cDNA，对目的片段进行PCR扩增、电泳纯化、T-A克隆及测序鉴定。

The recombinant proteins were purified with affinity columns by the 6 histidines tag or Glutathione-S-Transferase they were fused to.

并根据所表达融合蛋白的6个组氨酸或谷胱甘肽-S-转移酶标记进行亲和纯化。

METHODS: Recombinant human protein kinase CK2 α′ and β subunits were cloned, expressed and purified. The 2 subunits were mixed at equal molar ratio to reconstitute CK2 holoenzyme.

利用基因工程技术进行克隆、表达和纯化，获得重组人CK2的α′及β亚基，并在体外等摩尔混合构成CK2全酶。

METHODS: Recombinant human protein kinase CK2 α′ and β subunits were cloned, expressed and purified. The 2 subunits were mixed at equal molar ratio to reconstitute CK2 holoenzyme.

办法：利用基因工程技术进行克隆、表达和纯化，获得重组人CK2的α′及β亚基，并在体外等摩尔混合构成CK2全酶。

Recently many food derived fibrinolytic enzymes have been found in various traditional asian foods, such as Japanese Natto, Tofuyo, Korean Chungkook-Jang, soy sauce, edible honey mushroom, fermented shrimp paste, and Chinese douchi, and have been purified from these foods.

近年来在许多不同的亚洲传统酿造食品如日本纳豆、豆腐、泡菜、韩国大豆酱、食用磨菇酱、发酵虾酱和酱油等中均发现含有纤维蛋白溶解酶，并且从中分离纯化出这种酶。

OBJECTIVE To investigate the impurities of the hyodeoxycholic acid and its purification.

目的 研究猪去氧胆酸中的杂质成分和纯化方法。

RESULTS AND CONCLUSION Hyodeoxycholic acid was purified by recrystallization and column chromatography.

结果与结论从猪去氧胆酸中分离出一种主要杂质，建立了纯化猪去氧胆酸的方法。

Objective To explore how to improve the yield arid quality of rat islets with different purified methods.

目的 探讨不同纯化方法对提高大鼠胰岛收获效果的作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失