纯化的

Methods: Myoblasts isolated from rat muscle samples were cultured and purified clonally in vitro. The 3rd passage cells were incubated with medium including basic fibroblast growth factor and dimethyl sulfoxid.

体外分离培养大鼠成肌细胞并克隆纯化，培养至第3代时加入含有碱性成纤维细胞生长因子和二甲基亚砜的培养液诱导分化，进行RT-PCR分析和免疫荧光细胞化学染色鉴定。

To purify high purity lactoferrin from bovine colostrums,a fast separation technique was established by ion-exchange process.

从牛初乳中分离提取乳铁蛋白，建立快速分离纯化乳铁蛋白的工艺。

METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10^8/L were incubated in 12-well plate with a coverslip.

全骨髓法体外分离培养、纯化大鼠骨髓间充质干细胞，传至第4代时，按4×10^8 L^(-1)密度接种于置有盖玻片的12孔板内，制备细胞爬片。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

Methods: Encapsulate synthesized MG7 mimotope peptide into nanoemulsion using magnetic and ultrasonic technique. Dialyse method was used to sterilize, HPLC to determine encapsulation efficiency.

人工合成胃癌MG7-Ag的模拟表位多肽，用磁力超声法将其包封于纳米乳剂中，通过透析纯化，HPLC检测其包封效率。

Methods Fresh adult bovine tendon was dissolved with acetic acid and enzyme-digestion for type Ⅰ collagen. Then they were dialysed and purified for refined liquid collagen, which was cross-linked with glutaraldehyde.

取新鲜成年牛跟腱，用乙酸溶解－酶消化法提取Ⅰ型胶原，并进行盐析、透析、纯化得到精制的液态胶原，用戊二醛进行交联。

ResultsWith polyamide resin to absorb and purify, eluted with 40%,60%,70%,80% ethanol, good results were obtained.

结果通过聚酰胺树脂富集与纯化，并采用40%，60%，70%，80%乙醇梯度洗脱，可以使芦荟蒽醌衍生物得到较好的分离。

The combined 50% and 95% ethanolic elutes were concentrated to yield the purified effctive part.

总有效成分纯度为36.87%，说明采用D101树脂分离纯化虎杖有效部位是可行的。

Used the dynamic adsorption law to determine the resin adsorption purified in Liuhaoya the yellow ketones matter craft condition is: Uses the AB-8 adsorption resin,On the liquid volume is 100～150 mL approximately,The type fluid density is 1% approximately, elutes the fluid density is 70%,Speed of flow 1 mL/min.

采用动态吸附法确定了树脂吸附纯化柳蒿芽中黄酮类物质的工艺条件为：采用AB-8吸附树脂，上液量约为100～150 mL，样液浓度约为1%，洗脱液浓度为70%，流速1 mL/min。

As more and more bioactive substances have been isolated from endophytes, the natural products of endophytes show us plentiful diversity, and became an important source of new drug discovery.

众多内生菌活性成分已得到了分离纯化和鉴定，成为发现新型化合物的一个重要来源和途径。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。