纯化的

PAO was purified by Ni-NTA resin under denature condition and dialyzed to recover its native structure. After that, the enzyme activity was analyzed with chemical fluorescent method.

IPTG 诱导表达的重组PAO经Ni-NTA亲和层析纯化和透析复性后，化学荧光法测定酶活性。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料，经过过滤去除菌体，上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸，聚唾液酸沉淀用去离子水溶解，再用过滤去除变性蛋白质，滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后，再用过量乙醇沉淀出聚唾液酸，聚唾液酸沉淀用超纯水溶解，用层析柱进行分级纯化，收集洗脱液经过透析，冷冻干燥得聚唾液酸产品。

The steps were as follows: Acquiring superant from the culture medium by centrifugation, discarding waste residue by filtration, concentrating by hollow-fiber membrane, treating with salting-out, dialyzing the product of salting-out, treating the dialysate by vaccum freeze dryer, dissolving solid , using the method of gel filtration chromatography with Sephadex G-100/G-75, and proof-test by filter-paper chromatography ,then preparation by thin-layer chromatography was carried out.

建立了分离纯化该物质的有效途径：发酵液离心除菌→抽滤除杂→中空纤维过滤浓缩→盐析→透析→真空冷冻干燥→溶解→交联葡聚糖凝胶Sephadex G-100凝胶分离→交联葡聚糖凝胶Sephadex G-75凝胶分离→纸层析检验→薄层层析制备。

The vessels including clots were harvested and imaged. Cardiac muscle, liver, spleen, lung, kidney and so on were extirpated.

结果：(1)经亲和层析及PEG浓缩后的抗体浓度为2mg/mL，SDS-PAGE电泳证实纯化后产物纯度在95%以上。

Are firstly studied. And then the acidic extractions of algal cells is filtrated, analyzed for its nature and quantities.

同时还对渤海天津海域2005年棕囊藻赤潮水样中棕囊藻分离纯化与培养进行了初步的试验性研究。

Objective To optimize the production procedure for inactivated purified rabies vaccine for animal use.

目的 优化兽用灭活纯化狂犬病疫苗的生产工艺。

After some incubation time, cells are lysed and the protein is purified from the extract.

在经过一段时间的培养后，将细胞裂解，蛋白质可从裂解液中纯化出来。

The second passage was obtained after the cell overgrew the bottom of the culture flask and purified by trypsinization and adherence repeatedly methods.

方法1。用组织块贴壁培养法获得原代卵巢癌CAFs和卵巢NFs，细胞铺满培养瓶后首次传代，通过胰酶消化法和反复传代法进行细胞的纯化；2。

Escherichia coli leucyl-tRNA synthetase was overproduced and purified from an overexpression strain.

采用含pTrc-99B/leuS2转化子的高表达菌株，简便快速地提取、纯化大肠杆菌亮氨酰-tRNA合成酶。

BACKGROUND: Mesenchymal stem cells are few in bone marrow, and need to be isolated, purified and proliferated in vitro.

背景：间充质干细胞在骨髓中的含量很低，需进行体外分离纯化和大量增殖。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。