纯化的

After an acceptable antibody titer was observed,the animals were exsanguinated and antis.

通过兔颈部采血、抗血清纯化，得到的抗邻苯二甲酸二丁酯多克隆抗体IgG冻干后，分装在-20℃低温保存。

All mice were immunized for three times with an interval of two weeks. The mice were challenged with（40±1） cercariae of S.

每鼠肌肉注射相应的纯化质粒DNA 100 μg，每隔3周免疫1次，共3次。

The metal chelated chromatography used for purification of expressed CDB3 was simple and specific.

金属螯合层析纯化重组蛋白CDB3的方法简便，特异性好。

The peptidyl-resin was cleaved by...

纯化后的肽用HPLC进行纯度分析。

In this study, we cloned the BFV gag gene into prokaryotic expression vector pET28a and purified the denaturalized Gag protein. The protein was used to immunize BALB/c mouse to produce antiserum, which could specifically recognize the BFV Gag protein in BFV-infected cells through western blot assay.

本文将BFV gag基因克隆入原核表达载体pET28a中，纯化得到了变性的Gag蛋白，用于免疫BALb/c小鼠获得抗血清，进行了Western blot杂交和免疫荧光实验。

Both fusion proteins were purified under denaturing conditions which favor the transduction of proteins across the plasma membrane.

这两种蛋白皆是在变性的条件下纯化，这是为了利於蛋白穿透细胞膜。

At last, we tried to purify by Ni2+-chelating agarose column in native conditions and denaturing ones.

最后，在自然条件与变性条件下，应用His·bind Ni2+柱对融合蛋白进行了分离与纯化，初步得到了一条略小于20kDa的条带。

What was derided by some mathematical purists is now considered essential methodology in some areas.

什么是嘲笑一些数学纯化现在认为是至关重要的方法在某些领域。

The a-glucosidase inhibitor was extracted and purified from the fermentation liquid of Bacillus sp. GI-6, by means of precipitation of ammonium sulfate, dialyses, ion exchange chromatography and gel filtration.

2从B.sp.GI-6的发酵液中提取分离α-葡萄糖苷酶抑制剂，利用硫酸铵盐析、透析、离子交换层析、凝胶过滤等方法进行纯化，初步分离得到α-葡萄糖苷酶抑制剂。

The protein purified with Ni-NTA column was denatured by 8 mol/L urea and dialyzed for refolding.

Ni-NTA柱纯化后的特异性蛋白经8 mol/L脲素变性，梯度透析使蛋白质复性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。