纯化的

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。

MethodsThe ethyl acetate fractions from MeOH extracts of the spermoderm of Xanthoceras sorbifolia were partitioned and purified by means of several separation methods.

方法采用多种分离技术对文冠果种皮甲醇提取物的醋酸乙酯部位分离纯化，根据其理化性质和光谱数据进行结构鉴定。

ObjectiveTo study the techology for isolation of the total flavonoids from Spirodela polyrrhiza L.

目的探讨大孔吸附树脂法纯化紫萍总黄酮的工艺。

The purify serum were obtained by caprilic acid-ammonium sulfamate precipitation.

制备的血清抗体采用辛酸-硫酸铵分步沉淀法纯化。

Take the 10~12ml single-valve capacity as the example, with flings turns the head to separate evenly, 36.000rpm 60 the hour, turns the head with the angle type to separate 45, OOOrpm 36 the hour, the former including adds the deceleration to use in common 130,000,000 revolution of actuation department lives, the latter must use 100,000,000 revolution of actuation department lives, this was 100 ~ 20,000,000,000 transfers to at that time the supercentrifuge total life looked, without doubt each time tests the expense to be excessively high, in addition the CsCl amount used are many, price expensive and so on factors, causes this kind of separation purification work to become the very expensive experiment.

以10~12ml单管容量为例，用甩平转头分离，36.000rpm 60小时，用角式转头分离45，OOOrpm 36小时，前者包括加减速在内共用去1.3亿转驱动部寿命，后者也要用去1亿转驱动部寿命，这对当时超速离心机总寿命为100~200亿转来看，无疑每次实验费用过高，加上CsCl用量多、价格贵等因素，使这类分离纯化工作成为非常昂贵的实验。

Purification and Partial Biological Functions of β-l, 3-glucan Recognition Protein from Tenebrio molitor (Coleoptera: Tenebrionidae) Larvae

CEPS 思博网--文章书目；-1页数：6 需求点数：24 电子全文：请登入。篇名黄粉虫β-1，3-葡聚糖识别蛋白的分离纯化及部分生物学功能

A thermophilic strain CW2 was isolated and purified from the soil around a hot spring in Chang-bai mountains.

从长白山上一个温泉周围的土壤中分离、纯化出一株高温菌CW2。

After purification, ADH2 activity was tested in vitro and toxicologic test was done in mouse.

将该蛋白纯化后，在体外进行活性检测和小鼠体内进行毒理试验，检测ADH2的酶活性。

Forty-nine isolates of Trichoderma were collected and isolated from spawn and fruiting body of edible fungi.

从木霉污染的食用菌菌筒和子实体中分离纯化了49株木霉菌株。

In this thesis, the tripeptide GHK has been synthesized by liquid-phase method and octpeptide Eptifibatide by solid-phase method. Coupling agents and purification problem have been studied in the process of peptide synthesis.

本论文分别采用液相法和固相法合成了具有药用价值的三肽GHK及八肽Eptifibatide，研究了多肽合成过程中缩合剂及纯化等问题。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。