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Five bacteria strains were isolated from the intestine of Pieris larvae by traditional methods of separation, purification and cultivation.

从自然种群的菜青虫肠道环境中按传统分离、纯化、培养,获得细菌5个菌株,对其菌体形态、染色反应、培养性状、生理生化反应进行了系统研究。

Objective To extract and purify active polypeptides from the pilose antlers treated by various procedures and study their immunological function.

目的 提取并分离纯化不同加工方法处理的梅花鹿鹿茸活性多肽,并对其免疫功效进行初步研究。

Fifty-two protein peaks in the sera were significantly different between 57 patients with tuberculous pleuritis and 70 controls(P<0.01).The protein peak at m/z 5643 was highly expressed in 47.4%(27/57) sera from the patients with tuberculous pleuritis,and lowly expressed in 4 sera from the patients with non-TB pleuritis.

因此,选择5643 m/z蛋白作为潜在的诊断标志物,经RP-HPLC分离、纯化,并利用MALDI-TOF-MS及LC-MS/MS进行鉴定,5643 m/z目标蛋白质是粘蛋白,又称为α-1-酸性糖蛋白。57例结核性胸膜炎患者血清和70例对照血清蛋白指纹图谱比较,有52个差异蛋白峰(p<0.01)。

The aim of the research was to provide the experimental basis for the purification and the comprehensive utilization of the Ligninolytic enzymes from Pleurotus eryngii-Co60-7 strain.

[目的]为Pleurotus eryngii-Co60-7木质素降解酶的分离纯化和综合利用提供试验依据。

Cyclization: cyclization occurs at 100℃in polyphosphoric acid for 4h in the mass ratio of 3-(4-methoxyanilino) propionic acid to PPA with 1:8, and 6-methoxy-2,3-dihydro-4-quinolone is formed in 50.5% yield with a method of rapid chromatographic column, c.

成环反应:质量比[3-(4-甲氧苯氨基)丙酸∶多聚磷酸]=1∶8,反应温度为100℃,反应时间4h,柱层析纯化后6-甲氧基-2,3-二氢-4-喹啉酮的收率为50.5%;c。

The separation purification and purity identification of polysaccharose is difficult because of its complicated structure so it requests effective separating methods and high sensitivity detection.

由于多糖类药物结构极其复杂,所以其分离纯化以及纯度鉴定比较困难,因而必须寻求高效分离和高灵敏度检测的方法。

So the ability to purify, culture, and manipulate these postembryonic stem cells would provide investigators with an invaluable cell source to study cell and organ development.

因此,对于研究发育生物学而言,能够分离纯化、培养和有效利用胚胎后亚全能干细胞,无疑具有非常重要的意义。

The fusion protein was prepared and purified to feed larvas of Prodenia litura and Phanerotoma planifrons to evaluate its insecticidal effects.

对融合蛋白进行纯化,通过口途径饲喂瓜绢螟和斜纹夜蛾幼虫,以检测目的蛋白对两种虫的毒杀作用。

In experiment 1, a 5 weeks folate depletion protocol was employed, using 13-week old Hyline pullets assigned to receive a semi-purified folate-deficient diet or a commercial corn-soybean meal based diet supplemented with 1 mg/kg pteroylglutamic acid.

试验一:以叶酸缺乏之半纯化饲粮餵饲十三周龄之新母鸡,为期五周,藉以了解新母鸡叶酸缺乏时,血液相及血液生化值的变化,以作为叶酸是否缺乏之指标。

The c oaventioaal method was improved by using agarose as supporting medium instead pured agar and adopting pre-electrophoresis in order to make it more sensitive.

笔者在常规方法的基础上,做了些改进:用琼脂糖代替纯化琼脂;并先将诊断血清加在阳极端孔内,预电泳15—20分钟,然后将待检标本加入阴极端孔内继续电泳50分钟。

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