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The polysaccharides were extracted from Morinda officinalis How by pretreatment of cellulose,and were further purified by ion exchange and gel filtration chromatography.

采用纤维素酶水解方法提取巴戟天粗多糖,并通过离子交换和凝胶过滤层析对得到的粗多糖进行了分离纯化。

Immunoaffinity purification of the 47kD actin-binding protein on a MoAb1 matrix yielded a multimolecular complex composed of actin and two other proteins (MWapp 180kD, 71kD).

在MoAb1基质对47KD肌动蛋白结合蛋白亲和纯化,得出一个由肌动蛋白和其它二个分子量约为180KD及71KD结合的复合物。

It can induce complete immune responses, provide heterologous cross protection, and can be easily prepared as multivalency vaccine. On the other hand, compared with traditional vaccines, live attenuated Salmonella typhimurium S.

而与传统疫苗相比,活减毒鼠伤寒沙门菌这一新型口服疫苗载体释放系统亦具有无需纯化抗原,无需佐剂,可避免抗原在胃内的降解和变性等优点。

The antioxidant activity of the lipid-soluble epigallocatechin-3-O-gallate-4'- myristate in soybean salad oil were investigated and compared in AOM t.

通过酰化反应得到EGCG肉豆蔻酸酯,利用高速逆流色谱对反应产物进行分离和纯化,并进行了元素分析、MS和1H-NMR等表征,同时用活性氧法考察了其在大豆色拉油中的抗氧化活性。

Distribution and isolation of cellulolytic myxobacteriaWe assayed distribution of and isolated cellulolytic myxobacteria of several environmental types of samples, i.e.

为了更深入地研究和开发溶纤维素粘细菌资源,本文在溶纤维素群粘细菌的分离纯化、生态分布、系统分子分类、降解纤维素机制进行了初步探索。

The present doctoral dissertation was carried out to study the chemical constituents isolated from stems of two medicinal schisandraceous plants.34 compounds were isolated from the ethanol extracts of the stems S.henryi collected in the Jiangxi province by various column chromatographies.27 of them were identified on the basis of spectral analysis.There are 18 lignans,(including 5 dibenzocyclooctadienes,6 aryltetralins,2 tetrahydrofurans,3 diarylbutanes,2 other lignans),3 flavanols,2 glyceroesters,1 naphthoquinone,1 xanthine and 2 steroids,of which 6 compounds were new compounds,and 7 compounds were isolated from this family for the first time.

采用多种色谱技术进行分离纯化,从翼梗五味子藤茎的乙醇提取物中分离到34个化合物,综合运用现代波谱学技术鉴定了27个化合物,包括木脂素类化合物18个(其中联苯环辛烯类木脂素5个,芳基四氢萘类木脂素6个,四氢呋喃类木脂素2个,二芳基丁烷类3个,其他类型木脂素2个),黄烷醇类化合物3个,甘油酯类化合物2个,萘醌类化合物1个,黄嘌呤类化合物1个,甾体类化合物2个,其中,5个为新化合物,7个为本科首次报道。

Objective To express and characterize the C terminal fragment of HEV ORF2. Methods The gene encoding C terminal 128 amino acid fragment of HEV ORF2 was inserted into nonfusion expression vector pBV220. The resultant pBVORF2.3 was transformed into E.coli strain DH5α.

用pBV220载体进行抗原的非融合表达,包涵体经变性凝胶过滤层析及离子交换层析纯化后透析复性,以Western blot、ELISA、动物免疫与抗原捕获RT-nPCR等方法研究其免疫学特性。

In this study, the effects of culture conditions on the growth of recombinant E. coli BL21 (DE3) and the expression of subunit protein of Pasteurella multocida toxin 1 (Tox1) were investigated in both shaking flask and fermentor.

摘要 本研究探讨携带有猪萎缩性鼻炎次单位毒素Tox1基因之重组大肠杆菌大量表现重组蛋白质的最适条件,并对Tox1进行部份纯化。

A trypsin inhibitor was isolated and purified from Phaseolus vulgaris, and partial characterization of PVTI was studied.

本论文以菜豆的一个栽培品种紫花芸豆为材料,从中分离纯化胰蛋白酶抑制剂PVTI,并对其部分性质进行了研究。

The process parameters of the separation and purification of shikimic acid from Picea meyeri with ion exchange resin were established.

建立了离子交换树脂分离纯化白扦中莽草酸的工艺条件和参数。

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