纯化的

The immunological competence of the expressed protein was tested by means of Western blotting and enzyme-linked immunosorbent assay, and its biological activity was assayed by chicken chorioallantoic membrane and Matrigel angiogenesis assay.

酶联免疫吸附和Western blot技术检测纯化的VEGF165蛋白免疫学活性，鸡胚尿囊绒毛膜实验和matrigel血管形成实验检测其生物学活性。

The purified cTnⅠ was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.

纯化的cTnⅠ采用十二烷基硫酸钠－聚丙烯酰胺凝胶电泳和免疫印迹法进行鉴定。

Optimization of the extracting method of Chlorogenic Acid from Flos Lonicerae was studied by three different methods of ultrasonic extraction, sorbitic extraction, and improved method of sorbitic extraction.

采用超声波法、索氏法、改良索氏法3种方法分别从金银花中提取绿原酸，用紫外光谱法和高效液相色谱法对其进行定性定量分析。同时用金银花乙醇提取物及纯化的绿原酸对乳房炎主要致病菌进行抑菌试验。

Methods To do the immunoblotting test of the only Ⅱ fimbriae type of mutant strain of T14V.

采用免疫印迹实验对纯化的Ⅱ型菌毛进行分子质量检测。

Objective:To develop a linear algebra model to imitate the process of the allele homologizing within a closed inbreeding population.

目的：试建立线性代数模型，以拟合封闭近交群体内等位基因纯化的过程。

BALB/C mice were immunized with the purified PrV of MIN A isolate,mouse spleen cell were fused with myelomas NSo.Culture supernatants of hybridomas were screened by indirect ELISA and immobilization ELISA,positive colonies were cloned three times by limited dilution.

用纯化的伪狂犬病病毒免疫BALB/C小鼠，取脾细胞与NS_0骨髓瘤细胞融合，经间接ELISA和固相化ELISA筛选，3次有限稀释法克隆，获得了1株能稳定分泌抗伪狂犬病病毒单克隆抗体杂交瘤细胞株，命名为OH_6。

Results 61 mg IgY was extracted from one egg. The results of SDS-PAGE and Western blotting demonstrated that the IgY contained one major protein band with molecular weight of 130 000 and could be recognized by SEA.

结果 海兰母鸡经SEA首次免疫后35 d，每枚蛋经提纯后可得到约61 mg抗体，经SDS-PAGE和Western blotting分析，纯化的IgY有1条主要蛋白带，相对分子质量为130 000，并可被SEA识别。

Prokaryotic expressed protein products of candidate cDNAs were purified by SDS-PAGE and used as immunogens to prepare murine antisera.

5利用SDS-PAGE分离原核表达产物，并以纯化的表达产物为免疫原免疫小鼠，制备抗血清。

C4 antisera was obtained by immunizing rabbits with C4 antigen.

用纯化的 C_4免疫家兔制备抗 C_4血清。

Bioassay analyzing results showed that recombinant protein could catalyze the transfer of the amino group.

生物活性分析表明，纯化的重组天门冬氨酸转氨酶具有氨基转移活性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失