纯化的

Further purification was performed on a column of Q-sepharose FF, and the 50mM elute contained the active DM650 with a purity of about 95%.

复性后的蛋白用Q-Sepharose FF离子交换层析进行纯化，50mM NaCl洗脱峰中含有目的蛋白，纯度可达95％。

Using NaCl and Na2HPO4 in turn to elute bound alkaline phosphatase, a 65-fold purified enzymatic preparation was obtained in one step with a yield of 89%.

用NaCl和Na2HPO4进行阶段洗脱，可使酶的纯化倍数一步达到65倍，活力回收率达到89%。

Objective The polysaccharides from fruiting bodies of Hericium erinaceus were isolated, purified, and characterized.

目的对猴头菇子实体中的多糖进行分离、纯化和组成分析。

The separation and purification techniques of chlorogenic acid and flavonoids in Chinese medicine Eucommia ulmoide oliver were tested.

试验研究了杜仲绿原酸、总黄酮的分离纯化工艺。

4 Mg of the purified protein were obtained from 1 liter of bacterial culture.The results showed that rhCaM could extracellularly stimulate the proliferation of suspension-cultured cells of Angelica dahueica in the low density of initial cell culture.

每1 L菌液经Phenyl-Sepharose CL-4B 疏水亲和层析法纯化可获CaM纯品3～4 mg.rhCaM对白芷细胞增殖作用影响的研究结果表明，rhCaM在低细胞密度培养条件下对白芷细胞有促进增殖作用，效果高于标准植物CaM。

MethodsMicrowave-assisted flow and macroporous absorptive resin were employed respectively in the extraction and purification processes. The content of gentiopicrin in the fractions was evaluated by HPLC.

方法以龙胆苦苷含量和解吸率为指标，采用微波辅助萃取、大孔树脂吸附纯化和高效液相色谱法测定龙胆苦苷的含量。

ConclusionUnder the above optimum conditions the content of gentiopicrin was 34.57mg·g-1 from this batch of Gentiana scabra Bunge.

在优化的萃娶纯化条件下制得这批龙胆原料龙胆苦苷平均含量为34.57mg·g-1。

MethodsMicrowave-assisted flow and macroporous absorptive resin were employed respectively in the extraction and purification processes. The content of gentiopicrin in the fractions was evaluated by HPLC.

方法以龙胆苦苷含量和解吸率为指标，采用微波辅助萃娶大孔树脂吸附纯化和高效液相色谱法测定龙胆苦苷的含量。

The gene of human proinsulin mutant (A21Asn→Gly,B17Leu→Ile,B31Arg,B32Arg,GIRR ) was synthesized and recombinated to the fusion protein expression vector pTXB1 and expressed in E.coli BL21(DE3) with high protein level.

构建人胰岛素原突变体GIRR（A21Asn→Gly，B17Leu→Ile，B31Arg，B32Arg）基因的原核融合表达载体并进行表达和纯化，进一步对其性质进行研究。

Gymnemic acid was greatly refined by resin.

通过大孔吸附树脂，匙羹藤酸得到了较好的富集和纯化。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。