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Plant Virology 2 S T. H. Chen, FThe course will give conventional and molecular level of plant viruses in order to help the students to get much theoretical and practical knowledge about plant virology. The contents cover the molecular structure of virion and genome, virus isolation, dentification and purification, transmission, variation,ecology, nomenclature and classification, diagnosis and control.

本课程除传统植物病毒学范畴外,并涵盖从分子层次讨论植物病毒之构造,引起之植物病害,田间发生及防治,期能达到理论与实务并重;内容包括植物病毒分子层次的构造及核酸组成,病毒分离、鉴定与纯化,在寄主体内之增殖及转移,媒介传播、遗传及变异,其引起之病害及田间发生生态与防治。

During the incubation of purified α-synuclein with fructose or glucose, it was observed that the protein intrinsic fluorescence at 308 nm decreased while the fluorescence of glycated derivant at 447 nm increased.

将纯化后的α-synuclein分别与果糖和葡萄糖孵育,通过内源荧光、非酶糖基化衍生物特征荧光、圆二色光谱以及电子显微镜等技术进行检测发现:α-synuclein 与还原糖共同孵育后,308 nm内源荧光强度明显降低,同时在447 nm产生一个非酶糖基化衍生物特征荧光。

Three intrachain disulphide bonds have been found in porcine intestine peptide.They locates in the membrane of mitochondria in yeast.

尽管因种属不同在一级结构上略有差异,但其N端脯氨酸密集,22、23、25、35、44和54位点为6个半胱氨酸,从猪小肠纯化肽的3对链内二硫桥键已定位。

Though their primary structure are different with their species,they all have rich proline exists in N-terminal and 6-Cys locates in 22,23,25,35,44 and 54.Three intrachain disulphide bonds have been found in porcine intestine peptide.

尽管因种属不同在一级结构上略有差异,但其N端脯氨酸密集,22、23、25、35、44和54位点为6个半胱氨酸,从猪小肠纯化肽的3对链内二硫桥键已定位。

Though their primary structure are different with their species,they all have rich proline exists in N-terminal and 6-Cys locates in 22,23,25,35,44 and 54.Three intrachain disulphide bonds have been found in porcine intestine peptide.

尽管因种属不同在一级结构上略有差异,但其N端脯氨酸密集,22、23、25、35、44和54位点 6个半胱氨酸,从猪小肠纯化肽的3对链内二硫桥键已定位。

According to different acidity of free Flavonoids in Resina Draconis, different acidic lavonoids were extracted with the pH gradient extraction method from ethyl ether.

根据血竭中游离黄酮存在着酸性差异,采用溶剂除杂,转溶于乙醚,再用pH梯度萃取得到不同酸性的黄酮,进而对弱酸性组分分离纯化得到2个单体化合物。

The results showed that the-epicatechin-3-O-β-D-allopyranoside extracted from the rhizoma of Drynaria fortunei J.

本实验将从槲蕨根茎经纯化分离出之-epicatechin-3-O-β-D-allopyranoside,已於本实验室证明对骨母细胞有促进增生的作用。

Methods The constituents in the marcroporous adsorption resin 50% EtOH eluent of Drynaria fortunei were isolated by a combination of silica gel, Sephadex LH-20 and ODS chromatographies and their structures were identified by spectral methods.

利用硅胶、Sephadex LH-20、ODS柱色谱及制备型HPLC等方法进行分离纯化,根据化合物的理化性质及波谱数据鉴定结构。

To identity the strains of Eimeria conserved in our laboratory are purebred,11 strains of 6 Eimeria species were separated by single-oocyst method and infected chicken of 1—5 days old.

为了建立鸡球虫纯株,选用1~5日龄雏鸡,采用琼脂块单卵囊分离法对实验室保存的6种11株鸡球虫进行纯化,并选用2周龄雏鸡,对每个虫种/株选取1个单卵囊分离物进行增殖和种类鉴定。

The studies on separation of antibacterial substances and the identification by GC-MS indicate that: Eleocharis Tuberoses peel contains cholesterol, organic acids, eaters and flavonoids four types of active ingredients, and 11kinds of active material.

对荸荠皮抑菌物质纯化后进行的GC-MS鉴定结果显示:荸荠皮抑菌物质中含有甾醇类、有机酸类、酯类和黄酮类4类活性成分共11种活性物质。

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