纯化的

It is the first time to clone the BiVP gene from the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell. The purified recombinant protein fulfilled the needs of further studying the bioactivity of this protein.

首次在红光熊蜂中克隆到蜂毒蛋白酶基因，在昆虫杆状病毒表达系统中进行了表达，并对表达产物进行了纯化，为深入研究BiVP的生物活性奠定了基础。

Methods The BiVP gene was screened by the ESTs technology and cloned by PCR method from the cDNA library of the bumblebee, Bombus ignitus. It was reconstructed to form the baculovirus transfervector pBac1-BiVP and expressed in Sf-9 insect cell. The recombinant BiVP was purified by the fast protein liquid chromatography system.

利用ESTs从红光熊蜂cDNA文库中获得BiVP基因，以PCR技术扩增出BiVP的完整cDNA，并构建病毒表达载体PBac1-BiVP，通过昆虫杆状病毒表达系统，在Sf-9昆虫细胞中进行表达，表达产物用快速蛋白液相色谱系统进行纯化。

Ammonium sulfate,caprylic acid precipitation and ion exchange chromatography methods were used to purify McAbs against antigens of H37Rv strain.

单克隆抗体的纯化采用硫酸铵沉淀法、辛酸沉淀法和离子交换法。

It has been reported that F185K and C280S mutations of HIV1 integrase would improve the enzyme solubility, and the catalytical activity of the enzyme was the same as that of the wildtype enzyme in vitro.

coli中进行整合酶基因表达，SDSPAGE鉴定表达产物，亲和层析纯化蛋白，酶联免疫吸附实验方法测定整合酶的生物学活性。

Polysialic acid could form complex with cetyl pidinium chloride specifically and was thus used for the separation of PSA from E.

利用氯代十六烷基吡啶对聚唾液酸有特异性的络合效应来进一步分离纯化聚唾液酸。

The crude product was treated with Cetyl trimethyl ammonium bromide in order to separate the neutral glycoprotein and the acidic glycoprotein .

本文采用季铵盐沉淀法对粗产品进行部分纯化，分离其中的中性糖蛋白和酸性糖蛋白，最佳分离方案为：pH=7.0，样品浓度为1％，CTAB浓度为3％。

Objective: To establish a technical process for the purification of flavonoids from Chaenomeles speciosa Nakai and provide scientific reference for industrial manufacture.

中文摘要：目的：研究大孔吸附树脂分离纯化资木瓜黄酮工艺，为资木瓜黄酮的工业化生产提供依据。

The optimum condition was determined via orthogonal experiments, and the result shows that the technology of bile extraction and purification adopts 10L chromato bar with 3L bile, gel silica as fixed phase, the mixed chloroform and isopropanol as mobile phase, gradient elution with mobile phase (the mobile phase ratio in extraction of cholalic acid and glycocholic acid is 4:1 , and then becomes 3:2 in extraction of glycocholic acid and glycocholic acid), and eluting velocity of 3～5mL/s .

采用正交试验筛选了柱层析最佳条件，以容积为10L的层析柱进行分离纯化，固定相为硅胶，流动相为氯仿和正丁醇混合溶剂，一次装样量3000mL胆汁，流动相采用梯度洗脱，先按4：1比例分离胆酸和甘氨胆酸，然后按3：2比例分离甘氨胆酸和牛磺胆酸，流动相洗脱速度为3～5mL/s。

ConclusionThe AB-8 resin is suitable for separation and enrichment of chromone glycoside in extract of Yupingfeng.

结论AB8大孔吸附可用于玉屏风提取物中色原酮苷活性成分的分离纯化。

Here, a new method for large-scale preparation of Cinnamomin, a RIP from Cinnamomum camphora was established.

本文对香樟Ⅱ型核糖体失活蛋白Cinnamomin进行了大规模的抽提和纯化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。