纯化的

Adenosine was used as the raw material, dissolved in triethyl phosphate and then esterified with thiophosphoryl chloride under 0~5℃ for 8h without water and oxgen, in alkaline condition provided by 2,6-dimethylpyridine, introducing thiophosphoryl ester to adenosine.Then superfluous triethylamine was added and reacted under 5℃ for 1h to synthesize rough 5'-AMPS.The unpurified product was appled to a DEAE Sephadex A-25 column and eluted with triethylammonium bicarbonate.

用腺苷为起始原料，溶解于磷酸三乙脂中后，在无水无氧环境中及2，6-二甲基吡啶提供的碱性条件下与三氯硫磷以0~5℃下发生酯化反应8h，将硫代磷酸酯引入腺苷，然后再与过量三乙胺在5℃下反应1h，合成粗产品，再用碳酸氢三乙胺作洗脱液，过DEAE Sephadex A-25凝胶柱纯化。

Sequences for each individual were assembled and edited using ESEE 3 0 s. A distance matrix was computed using program DNADIST of PHYLIP(3 57). Unrooted maximum likelihood trees were calculated from program FITCH.Results The amplified CO1 gene of the snail was a fragment of 638 bp in length.

在该地区选 7个点(上游 4个点，下游 3个点)采集钉螺，用CTAB法提取钉螺基因组DNA ，PCR方法扩增CO1基因，纯化后测序，运用ESEE软件排序并比较变异位点，观察各点钉螺的CO1基因单倍体型，运用PHYLIP软件计算遗传距离，绘制基因进化树。

OECs were cultured in the olfactory bulb of newborn Wistar rat. OECs suspension was infused into the lateral cerebral ventriculus of EAE rats, and the saline with the same quantity was infused in pseudo-operation group. Afterwards, motor-function grade, histo-pathological change and expression of apoptosis of neuron were observed.

培养、纯化OECs后，移植组将OECs悬液注入EAE大鼠的侧脑室；假手术组按以上方法注入等量生理盐水；观察二组大鼠运动功能评分、组织病理学变化以及神经元细胞凋亡表达情况。

Because the physical and chemical properties of 5'-Cytidine triphosphate,5'-Cytidine disphosphate and 5'-Cytidine monphosphate are very similar, and thereare many impurities in the solution of zymolysis, the separation of down stream isgreatly difficult.

由于三种磷酸胞苷的理化性质很相近，而且三磷酸胞苷酶转化液中各种杂质含量较多，导致下游分离纯化很困难。

Their structures were detailedly identified on the basis of chemical methods (sugar composition analysis, glycosyl residue linkage analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, acetolysis, Congo Red experiment, etc.), spectral technologies 1D, 2D NMR, ESI-MS, IR, etc.

通过各种化学方法（糖组成分析，糖残基连接方式分析，部分酸水解，高碘酸氧化，Smith降解，乙酰解，刚果红实验等）、光谱方法（1D、2DNMR，ESI-MS，GC-MS，IR等）和物理化学方法（分子量分析，粘度分析，旋光度分析等）对各纯化多糖的化学结构及糖链构象进行了鉴定。

We first isolated CD4+CD25+ T cells isolated from nonautoimmune DBA2/NZW F1 mice and adoptively transferred to NZB/NZW F1 mice, which spontaneously develop lupus.

我们首先从正常老鼠(DBA2/NZW F1)分离纯化出CD4+CD25+ T细胞当作正常的调性T细胞，并且利用尾静脉注射移植到红斑性狼疮小鼠中。

To discover the method of enrichment and purification of total terpenoid components from Alisma Orientalis with macroporous resin.

探讨用大孔树脂富集纯化泽泻总萜醇类成分的方法。

The macroporous resin AB-8 could be effectively used to enrich and purify the total terpenoid components from Alisma Orientalis.

AB-8大孔树脂可有效用于泽泻总萜醇类成分的富集与纯化。

The mineral system of alite calcium barium sulphoaluminate with good binding characteristic was synthesized with chemical reagents as raw materials.

以分析纯化学试剂为原料，对以阿利特与硫铝酸钙钡为主导矿物的高胶凝性矿相体系进行了研究。

The AmkA, AmrA and AmrB had been purified with the His-Tag chromatography column.

其中，amrA，amkA和amrB基因的表达产物已经His-Tag亲和柱进行了纯化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。