纯化的

Objective To investigate the method of isolation and purification of epithelial cells of human eccrine sweat gland in vitro.

目的探讨建立人皮肤汗腺细胞体外分离及纯化培养的技术。

Methods Through digesting human skin with collagenase type Ⅱ, cells of human eccrine sweat gland were isolated.

通过酶消化法分离人皮肤汗腺，微量加样器在倒置显微镜屏视下吸取游离的汗腺组织移入Hank平衡盐溶液，经纯化后予以原代培养。

ABSTRACT Bacillus subtilis tryptophanyl-tRNA synthetase was expressed and purified by the aid of the high-level expr...

利用枯草杆菌色氨酰tRNA合成酶的高表达质粒pKSW1表达和纯化了枯草杆菌色氨酰tRNA合成酶。

Objective To clone and express Taenia saginata asiatica's Spef1like gene,and purify its protein.

目的 扩增、克隆和表达亚洲牛带绦虫Spef1Like基因全长cDNA，并进行表达产物的纯化。

Taenia saginata asiatica's Spef1like gene was successfully cloned and its expression and fusion protein was purified,which provides favorite conditions for further studies of the gene.

成功克隆了亚洲牛带绦虫Spef1Like基因，并表达和纯化了Spef1Like蛋白，为研究Spef1Like的功能及其疫苗等研究提供了基础依据。

With rarefied the albumen after is vaccine of oily emulsion of immune former preparation immune egg chicken, the result makes clear, CCK albumen can cause immune reaction inside gallinaceous body, tenability of antibody of the CCK in serum of chicken of the egg after strengthening immunity and yolk is in higher level.

以纯化后的蛋白为免疫原制备油乳剂疫苗免疫蛋鸡，结果表明，CCK蛋白可在鸡体内引起免疫反应，加强免疫后蛋鸡血清和卵黄中CCK抗体可维持在较高水平。

Thermolysis of DM-DMI bis-carbamate was carried out in 3wt % solution of diphenyl ether at 254℃ for 5-6 hours, and the yield of DM-MDI is 61％ after purification.

而在第二步骤的热裂解反应中，在3wt ％二苯醚溶液中，控制反应温度在254℃，经反应5-6小时即得到DM-MDI，经浓缩及纯化后主产物之产率为61％。

Objective:To optimize the technical parameters of enrichment and purification of the total glycosides in tree peony bark with macroporous resin.

目的：优选大孔吸附树脂富集纯化牡丹皮中丹皮总苷的最佳工艺参数。

Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF－9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7；以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达；用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

Igniarius were carried out. It will be of scientific significance in researching and developing rare medicial fungi and be useful for searching new natural antitumor medicine.The crude polysaccharides of P. igniarius PIP30、PIP60、PIP80 and PIWE1-10、PIWE10-100、PIWE100 were obtained by ethanol precipitation and ultrafiltrate respectively after hot water extracting.

首先对桑黄子实体多糖进行了分离纯化，通过热水提取、乙醇分级沉淀分离得到粗多糖PIP30、PIP60和PIP80，通过超滤分离得到粗多糖PIWE1-10、PIWE10-100和PIWE100；6种粗多糖经体外刺激淋巴细胞增殖实验表明均有体外刺激淋巴细胞增殖的作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。