纯化的

PAO polyclonal antibody was prepared by using recombinant human PAO protein which was purified by polyacrylamide gel electrophoresis as antigen to intradermally inoculate and immunize rabbit.

用制备性聚丙烯酰胺凝胶电泳分离纯化的重组人PAO蛋白，皮内接种日本大耳白兔以制备多克隆抗体。

Firstly, the method of isolation, culture and purification of neonate rat Schwann cells in vitro was investigated.

首先，探讨新生鼠雪旺细胞体外分离培养和纯化的方法。

Obtaining relative pure OECs through isolation and culture is the most important.

分离、培养、获得相对纯化的OECs是关键。

Objective:To study the extracting conditions and purification process parameters of Rheum.

目的：研究大黄提取纯化的工艺条件及参数。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

Polyclonal antibodies against the purified Lv from swordtail fish were produced in the mice.

用纯化的剑尾鱼Lv免疫大白鼠获得鼠源多克隆抗血清。

An indirect competitive enzyme-linked immunosorbent assay was established for detecting vitellogenin in the whole body homogenate of male swordtail fish.This technique was developed with antiserum of lipovitellinresistance as antibody and Vtg as antigen.

以卵黄脂磷蛋白(lipovitellin，Lv)抗血清为抗体，以纯化的卵黄蛋白原(vitellogenin，Vtg)为抗原，建立了间接酶联免疫吸附反应方法检测剑尾鱼雄鱼整体匀浆液中ρ。

Kombucha was fermented with pure strains of yeast Y1, lactobacillus R3 and acetobacter C5, and the technical conditions of fermentation were optimized.

采用自行分离纯化的酵母菌Y1、乳酸菌R3、醋酸菌C5进行纯种混合发酵生产红茶菌饮料，并对发酵工艺条件进行优化。

With the SDS-PAGE preparation electrophoresis, high quality insecticidal protein was purified from a unique Bacillus thuringiensis serovar japonensis strain HBF-1, which is highly toxic to larvae of Anomala exoleta and A.

以苏云金芽孢杆菌HBF-1菌株中分离纯化的高特异性杀虫蛋白作为抗原，免疫新西兰白兔制备出多克隆抗体，其效价可达到16000。

ABSTRACT Partially purified chlorophyll was used to prepare pheophorbide by chlorophyllase hydrolysis and selective acidic hydrolysis methods.

以初步纯化的叶绿素为原料，利用叶绿素酶水解、选择性酸降解两种方法制备脱镁叶绿酸。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失