纯化的

The purification and character of spectrum of red protein R(subscript x of Nostoc flagelliforme was studied in the paper.

研究发菜中一种红色蛋白R(下标 x的分离纯化及其光谱学性质。

Through four steps of column chromatography and electrophoresis purification, one kind of nuclease was obtained.

样品依次通过四步柱层析，PAGE-DNA功能胶纯化得到电泳纯的双胸蚓核酸酶样蛋白质纯品，命名为DNase E。2。

As a result, a series of 526 bp nucleotide sequences of partial control regions were obtained.

PCR产物经纯化后进行序列测定，得到了526 bp的核苷酸序列。

This paper introduces the features of ideal samples for analysis of in-situ-produced cosmogenic nuclide s of 10Be and 26Al.

着重介绍原地生成宇宙成因核素10Be和26Al理想样品的特点、野外采集注意事项、实验室分离与纯化以及加速器质谱测量用靶样制备等。

Preparation of separate astroglial and oligodendroglia cell cultures from rat cerebral tissue .

大鼠星形胶质细胞和少突胶质细胞的纯化培养与鉴定。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

I think it's a vast oversimplification.

这又不是我说的，这太过度单纯化了

This description is oversimplified, but I think it's a useful model towork with here.

这个描述过分单纯化了。但我想在这里它是个有用的模型。

Therefore, the method which we established in this paper was a good ways to extract TCA from ovine bile.

因此，该方法是从羊胆汁中提取纯化牛磺胆酸的有效方法。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。