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Results:92% purity of hirudin was obtained after the separation purification.

结果:经过分离纯化可以得到纯度为92%的水蛭素。

The purified proteins were used to immunize Balb/c mice and to generate hybridomas.

得到的包涵体蛋白经纯化后免疫Balb/c小鼠并进行细胞融合。

The expressed fusion protein and GST-VP3 were purified, and used as an immunogen to raise GST-VP3-specific polyclonal antibodies in male rabbits.

纯化表达的GST-VP2e融合蛋白,利用其作为免疫抗原,制备了兔抗GST-VP2e融合蛋白多克隆抗体,其效价达到1:512,000。

Aim To study the immunogenicity and safety of purified Vero cell rabies vaccine for human use.

目的研究人用狂犬病纯化疫苗的安全性和保护性。

The result showed that NS1 protein was highly expressed in a way of inclusion body.

用HisTrap HP Kit进行复性和纯化,获得了纯度较高、生物活性较好的NS1蛋白。

The P1 recombinant protein extracted and purified with B-PER GST Fusion Protein purification Kit was used as an antigen, and thallus component of full Mp was used as an antigen control to establish indirect ELISA experimental method to test and determine IgG antibody of serum samples from 40 normal blood donors and 51 clinical serum of partly certain and partly uncertain Mp infector.

以GST融合蛋白层析柱提取、纯化Mp P1重组蛋白做抗原,以全肺炎支原体菌体成分做抗原对照,建立间接ELISA实验方法,检测40份正常献血者血清标本和51份疑似MP感染临床血清标本的IgG抗体。

Objective To explore a simple and rapid method for the isolation and purification of minipig islet cells.

目的 探索一种简单快速分离纯化小型猪胰岛的方法。

The best fermentation condition was chosen, and the antibacterial substance was isolated and extracted.

选择了适合该菌株的发酵条件,并通过硫酸铵沉淀、Sephadex G-50柱层析、高效液相色谱和质谱分析,对抗菌物质进行了分离纯化,并研究了其部分性质。

Objective To investigate the chemical constituents from the seeds of Mirabilis jalapa. Methods Various chromatographic techniques were used to separate the chemical constituents.

目的 研究紫茉莉子的化学成分方法利用各种色谱技术进行分离和纯化,通过理化方法及NMR、IR和MS 等方法解析其化学结构。

Objective To select the optimal process parameters for refining total flavonoids from the leaves of Diospyros kaki using macroporous resin.

摘要目的筛选大孔树脂纯化柿叶总黄酮的最佳工艺,为后续研究提供基础。

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