纯化的

Euglobulin precipitation combined with gel filtration is a good method for purification IgM Mabs from ascites.

本文通过优球蛋白沉淀结合凝胶过滤法纯化腹水IgM型抗体，并与传统的硫酸铵沉淀结合凝胶过滤法相比较。

Application of McAbs: the ascites of McAb A_12B_4 were purified with euglobulin precipitation, and tagged with colloidal golds, then the immuno-gold filtration assay was developed to examine avian influenza virus. The results showed that the positive outcome was gained with avian H_9, H_7 and H_5 influenza virus.

单抗的初步应用：A1284株腹水经优球蛋白沉淀法纯化后与胶体金标记，应用于胶体金试纸条检测试验，金标A1284株单抗与H9、H7、H5三种亚型禽流感病毒反应结果呈阳性。

Coli strain stably and highly expressing recombinant Pseudomonas aeruginosa exotoxin A and for purification of expressed product.

目的 建立稳定、高效表达重组铜绿假单胞菌外毒素A的工程菌发酵及表达产物纯化工艺。

Methods Mesenchymal stem cells were collected from the femora of SD rats by density gradient centrifugation and identified by flow cytometric analysis and alkaline phosphatase staining.

方法采用梯度离心法，分离纯化SD大鼠的MSCs，流式细胞仪检测和碱性磷酸酶染色鉴定细胞类型。

The fixed carbon content in the sample can be calculated from the difference between two weightings.

在600℃灰化滤纸并纯化固定碳90 min，称重，然后在1000℃灼烧至恒重，前后两次重量之差值为固定碳的结果。

Macroporous adhesive resin column and Sephadex LH20 column were employed to separate and fractionate the crude extract and the structures of the main compounds were analyzed by HPLC-ESI-MS.

通过大孔树脂结合SephadexLH—20对芒果多酚粗提物进行分离纯化和分级制备，采用HPLC-ESI-MS对主要组分的结构做了初步分析。

Purified proteins were mixed with Freund's adjuvant and then were used to immunize rabbits.

将纯化后的GST-R185、GST-R150和GST蛋白免疫新西兰大白兔制备抗血清。

Fusarium oxysporum from wilt diseases in Guangxi hybrid bamboo were separated and purified, and the biological characteristics of the pathogen were studied.

分离纯化了广西杂交竹枯萎病原镰刀属尖孢镰刀菌，并研究了该病原菌的生物学特性。

Methods Chromatographic column coupled with gallinaceous ovomucoid was used to isolate and purify WGA.

先制备鸡卵黏蛋白的亲和吸附剂，然后用其纯化麦胚凝集素。

Immunize BALB/c mice with the produced GAMP-ADH-Pep10 conjugate after purification, determine the serum IgG level against GAMP by indirect ELISA, and analyze the immune suppression induced by carrier protein.

纯化后免疫BALB/c小鼠，用间接ELISA法检测小鼠血清中抗GAMP IgG抗体水平，并分析载体诱导的免疫抑制率。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。