纯化的

Methods Highly purified HCV Core, NS3, NS4, NS5 proteins were immobilized on solid matrix by biochip arrayer, the protein chip was used to detect the corresponding antibodies in human serum by chemiluminescent immunoassays.

利用生物芯片技术和化学发光技术将高度纯化的抗原HCV Core、NS3、NS4、NS5以特定微阵列固定在固相载体上，用化学发光免疫法检测抗HCV Core、抗HCV NS3、抗HCV NS4和抗HCV NS5。

Huwentoxin-XVIIb1 (HWTX-XVIIb1) is a novel peptide neurotoxin, isolated and purified from the Chinese bird spider Selenocosmia huwena Wang. It contains 31 amino acid residues including 8 cysteines.

虎纹捕鸟蛛毒素-XVIIb1 (HWTX-XVIIb1)是从虎纹捕鸟蛛粗毒中提取、表达、分离纯化的一种新型多肽，它由31个氨基酸残基组成，含8个半胱氨酸。

Western Blotting experiments shown that the CspA protein of Bacillus stearothermophilus can be expressed under the condition of not only cold shock but also the induction of Chloromycetin.

用纯化的蛋白质免疫兔子获得抗血清，Western Blotting表明CspA蛋白于嗜热脂肪芽孢杆菌中不仅冷激时表达，而且在受到氯霉素诱导时也会表达。

He says of them that, during the generation, they were filled and purified by the Holy Ghost, and freed from sexual concupiscence.

他说，他们说，在一代人，他们被填补和纯化的圣灵，并摆脱性concupiscence 。

The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody.

纯化的融合蛋白免疫新西兰大白兔制备多克隆抗体。

Genome, and its expression products are located in the flagellar, cytoplasm and fertilization tubule. Cr. actin is partially purified from Cr. cells by the method of assembly-disassembly-circle of actin.

我们利用肌动蛋白本身所具有的聚合和解聚的性质，从衣藻细胞中部分纯化了衣藻肌动蛋白，纯化的肌动蛋白能和抗兔α-平滑肌肌动蛋白的单克隆抗体发生专一性很强的免疫交叉反应。

Purified GO had a molecular weight between 145, 000 and 162, 300, as determined by Sephadex G-200 filtration and SDS-PAGE of the enzyme as cross-linked with glutaraldehyde. This result showed that the holoenzyme was a tetramer, and no change occurred in molecular weight of the enzyme during the s〓orage. The active form of GO from brassica leaves showed a constant molecular weight during purification, so did that from O. sativa leaves during greening of etiolated seedlings, maybe acting as a stable tetramer.

用Sephadex G-200胶过滤和戊二醛交联后的SDS-PAGE法测得纯化的菜心叶片GO的分子量在145，000-162，300间，为四聚体；并且在放置过程中其分子量和聚合态均保持不变，为稳定的四聚体；进一步研究表明菜心叶片GO在纯化过程中和水稻叶片GO在黄化苗的转绿过程中，两种GO活性状态的分子量和聚合态也没有发生变化，均呈四聚体。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Western blots were performed to investigate the cross-reactivity of various labeled antibodies with serum proteins from other fish species. Results Immunoglobulins of five fish species were purified, including bighead carp, carp, argus snakehead fish, ricefield eel, sea bass. The titers of five rabbit anti-sera obtained up to 1:32 examined by agarose double immunodiffusion. Five kinds of purified rabbit anti-fish immunoglobulins antibodies were labeled with HRP and their activities were up to 1:10000 examined by EUSA. Four kinds of rabbit anti-fish immunoglobulins antibodies labeled with HRP could react with serum proteins from other fish species.

结果 纯化了鳙、鲤、乌鳢、黄鳝、鲈五种鱼血清免疫球蛋白，免疫双扩散法测定兔抗这五种鱼免疫球蛋白的抗血清效价均达到1：32，并对纯化的兔抗鳙、鲤、乌鳢、黄鳝、鲈五种鱼类免疫球蛋白的抗体进行了HRP标记，ELISA测定标记抗体的效价达到1：10000左右，Western blots显示标记抗体与部分其他鱼类免疫球蛋白之间存在不同程度的交叉反应。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失