纯化的

The opposite application comprises: with Macleaya cordata fruit or grass as material and ethanol as extraction solvent, using just the adsorption-elution technique of one-step to separate and purify the high-pure sanguinarine from Macleaya cordata raffinate with purity more than 60%.

吸附树脂的应用是以博落回果或草为原料，以乙醇为提取溶剂，仅通过&吸附-洗脱&一步简单工艺，即可从博落回提取液中分离纯化得到高纯度血根碱的提取物，产品中由博落回果纯化得到血根碱的纯度最高可达60％以上。

The application effects of enzyme preparation in mashing process and kinetics for degradation of β-glucan in wort was studied.

探讨了双水相纯化β-葡聚糖酶工艺，筛选适宜的纯化体系，最后研究了β-葡聚糖酶在麦芽糖化过程中的应用效果，并建立了降解β-葡聚糖的动力学方程。

For Os separation, solvent extraction (Br_2 or CCl_4) and distillation are two mainly effective techniques, and microdistillation is used to further purify Os.

Os的分离纯化方法有蒸馏法和Br2或CCl4萃取法以及微蒸馏法，它们是分离和纯化Os的有效方法，也是目前国内外大多数实验室所采用的方法。

Methods Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用套式聚合酶链反应方法对5例艾滋病患者血浆HIV-1 env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及核苷酸序列进行分析，并计算其核苷酸同源替换率与非同源替换率的比值。

Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用巢式PCR方法对5例艾滋病患者血浆HIV-1env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及氨基酸序列进行分析，并计算其氨基酸同源替换率与非同源替换率的比值。

The results showed that plenty of the purified DPV virions were obtained after the cell lysate was condensed by 5-fold by ultrafiltration,and the concentrated DPV fluid was ultracentrifuged with 300g/L sucrose cushion method and 300-600g/kg sucrose density gradient centrifugation methods.

结果显示，用切向超滤法将病毒培养物浓缩为原体积的1/5后，再采用300g/L蔗糖垫底超速离心和300～600g/kg蔗糖密度梯度离心的方法进行纯化，可获得大量的纯化病毒粒子。

The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train.

本申请的特征还在于洗脱被纯化多肽的方法及纯化系列中该方法的并入。

Suitable macroporous adsorption resin (MAR DM-21) was chosen to separate SDG from extract solvent system (KOH-ethanol-water) of flaxseed cake.

然后用大孔吸附树脂与TLC相结合的方法，探讨了亚麻籽中SDG的分离纯化，并根据HPLC分析结果确定了最佳的分离纯化工艺条件。

Objective: To optimize the technical parameters of separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins.

目的：研究大孔吸附树脂分离纯化三七花蕾中总皂苷的工艺条件及参数。方法：以三七花蕾总皂苷的吸附量和洗脱率为指标，筛选大孔吸附树脂，并研究所选树脂分离纯化三七花蕾总皂苷的吸附和洗脱条件。

The inclusion body of IFN-BLA was denatured and refolded by dialysis and purified by ion-exchange chromatography.

对包涵体的复性条件进行了摸索，建立了IFN BLA的复性及纯化方法，纯化后的蛋白产量约为45mg/L，纯度在90%以上。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。