纯化的

The stimulations of wheat tonoplast H +\|ATPase by both Mg 2+ and Ca 2+ were measured on the base of purification of the enzyme.

纯化小麦液泡膜H+-ATPase，测定了纯化的小麦液泡膜H+-ATPase 受Mg2+和Ca2+双重激活的特性及两种激活状态下酶的特性。

Unpurified protein. 2:Extract of cells infected with vaccinia virus 3:Extract of cells un-infected with virus. 4:Purified protein

层析纯化前的粗提物； 2：野型病毒感染的细胞粗提物；3：未经感染的细胞粗提物； 4：经纯化的蛋白

The purified immunoglobulins were effective in the inhibition of endothelial cell adhesion, proliferation and angiogenesis in chick chorioallantoic membrane in vitro, and in anti-tumor activity by the adoptive transfer in vivo.

将该抗体纯化后，纯化的免疫球蛋白可以有效地抑制体外内皮细胞的粘附、增殖和鸡胚绒毛尿囊膜的新生血管形成，体内过继输入治疗亦可引起抗瘤效果。

Using SPDP as a heterobifunctional crosslinker, R-PE isolated from Corallina officinalis and sheep anti-rabbit IgG purified by ion exchange chromatography as conjugated reagent, we prepared the phycoerythrin fluorprobes by two-step binding protocol.

以双功能试剂SPDP为交联剂，从珊瑚藻中纯化的R-PE和购买的离子交福建农林人学博1：学位论文2二换纯化的羊抗兔抗体为标记材料，利用两步标记法获得藻红蛋白抡疫荧光探针。

The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence ＋ serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁＋无血清条件培养液纯化的实验。

METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

The gradual process of allele homologizing in a closed inbreeding population is imitated quantitatively by using linear algebra model.

线性代数模型定量地模拟了封闭近交群体内等位基因逐步纯化的过程，同时能客观分析不同自交代之间的生物数学特征及内在联系，根据模型还能由初始条件预报纯化基因型的频率。

The target protein was purified under the native condition and the polyclonal antibody was prepared by immunizing rabbits.

因此，可以在自然条件下进行目的蛋白的纯化，并将纯化的蛋白对家兔进行免疫而制备多克隆抗体。

On the other hand, Coarse goods ofβ-carotene was purified by macroporous resin and the purification parameters wasdetermined by single factor experiment. The effect of ray, heat, preservative and usualmetal ions to stability ofβ-carotene was also researched.

另外，利用大孔吸附树脂对β—胡萝卜素的粗提液进行了纯化，通过单因素试验确定了纯化的最佳工艺参数，同时，将光、热、常见防腐剂苯甲酸钠、常见金属离子对β—胡萝卜素的稳定性的影响进行了研究。

At first, we investigated the optimal components of culture medium for thereafter purification. We found that the optimal culture medium could produce better yield of red-pigmented antibiotic when 1.5% fructose and 1.5% malt extract included, and incubated at 30℃ under shaking conditions for 6 days.

我们逐步由探讨培养基条件为始，以利后续的生产纯化的进行，发现以1.5%果糖与1.5%麦芽抽出物之液态培养条件下，以30℃震荡培养6天后，有最适红色抗菌物质之产率，并且可减少其他色素的产生，再试著将其纯化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失