纯化的

Using awater purification cartridge beyond its expiration date or failing to clean the system on aregular basis can lead to water with higher resistivity as well as microbial contamination.

设备所用的纯化柱填料过期或没有按照程序来清理纯化柱，可能会导致水的电阻率升高或被细菌污染。

The crude DNA pellets were recovered by dialyzing and then could be used for molecular operation such as amplification of 16S rRNA gene and digestion of restriction enzymes.The plasmid pUC19 was prepared and digested with BamHI.

粗提DNA用半透膜透析回收纯化，纯化后的DNA可以进行16sRNA基因PCR扩增，酶切等分子生物学操作，具有较高的质量。

The unfolded protein correctly refolded to the native conformation through the column, and was partly purified during the elution process.

其原理是将层析技术应用于蛋白质复性和纯化，使变性蛋白质在层析柱上折叠为正确的空间构象，在洗脱的同时实现部分纯化。

The mixed microbial populations from the bioleaching plant in Zijinshan Copper Mine is separated and purified,and the identification based on the molecular biological,morphologic and physiologic methods of the microbe depurated and cultured are conducted by polyphasic taxonomy and phylogeny analysis with application of the 16S rDNA sequences and DNA homology technology.

对福建紫金山铜矿生物堆浸系统中的浸矿微生物进行分离纯化，对得到的纯化培养物采用了多相分类与系统发育分析方法，应用16S rRNA基因全序列分析技术，进行分子生物学鉴定、微生物形态性征与生理生化代谢特征鉴定。

Finally, wetested its antibacterial activities by E.coli and S.aureus.Methods In this research, the recombinded gene was cloned into the secretion vector pTYB12 and expressed in the E.coli BL21DE3. We did the preliminary research on secretion-expression of the gene. During the purification procedure, we used the chintin beads to wash off the sundry protein, then dialysed the collecting liquid to get rid of the salinity and the other small molecular peptides.

将上述重组基因抗菌肽的氨基酸片段克隆并拼接到分泌表达载体pTYB12上转染大肠杆菌(E.coli BL21DE3)后进行分泌表达，破碎离心收集的诱导表达大肠杆菌，首次应用几丁质珠柱分离纯化系统进行分离纯化，分离洗脱杂蛋白后透析去除盐类物质及其它小分子肽，得到目的重组基因抗菌肽。

Including the soybean lecithin separation purification, gamma - the linolenic acid separation and the purification, as well as gamma - linolenic acid fat 质体 Bao He studies and so on, and carries on optimal using the orthogonal experiment to the above craft.

其中包括大豆卵磷脂的分离纯化，γ-亚麻酸的分离和纯化、以及γ-亚麻酸脂质体包合研究等等，并利用正交试验对上述工艺进行优选。

Coli, and purify rWHcAg under native condition by precipitation with saturated ammonium sulfate and CsCl gradient centrifugation; Immunize Balb/c mice with purified rWHcAg to prepare antiserum; Establish a competitive inhibition ELISA method to detect WHcAb in serum of Chinese marmots.

原核表达重组WHcAg，氯化铯密度梯度离心获得非变性的纯化蛋白；用该纯化蛋白免疫Balb/c小鼠制备多克隆抗体；建立竞争抑制ELISA方法用于检测旱獭血清中的抗－WHc。

In general, the SERRS enhancement can also get a contribution from a so-called "chemical" SERS enhancement mechanism, which is based on the specific interaction between the target molecule and the metal substrate.

六。对单壁碳纳米管的纯化工作进行了讨论，我们采用了物理和化学方法相结合的方式来纯化单壁碳纳米管。

Results:We hae screened out the best method of drawing the materials from OB,which is crushing directly. we also conside the immunoadsorption as the best method of puring OECS.Coating the petri dish is profitable to pure the cells. meanwhile the appropriate cells density inoculated is important for eentual purity of culture.

结果：我们从中筛选出最佳取材方法为直接挤压法，最好的纯化嗅鞘细胞方法为免疫吸附法，同时发现包被培养皿对纯化细胞意义重大，而高密度接种细胞对提高细胞的最终纯度也是非常有利。

It is a very important progress that a new procedure was developed using a re-formative gradient medium and by ultra-centrifugation for the isolation and purifi-cation of intact WSSV viral particles from the infected penaeid shrimp or crayfish.

在完整WSSV病毒粒子纯化技术上取得重要研究进展。采用改良的密度梯度离心介质建立了一套成熟的WSSV纯化方案。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。