纯化的

In order to simplify the procedure and obtain high quality gDNA, we alternate water-bath to dewax, extract gDNA from hepatocellular carcinoma embedded by paraffin, and amplify gene using HCC gDNA.

为了简化操作，获得更高质量的gDNA，我们采用水浴法替代二甲苯进行脱蜡，亲和层析纯化石蜡包埋肝细胞癌组织标本中基因组DNA，以纯化gDNA为模板PCR扩增看家基因。

Keywords: diketoreductase,isomeride,separation and purification,statins chiral side-chain expression, photoaffinity labeling probe

通过DEAE-Sepharose离子交换柱及Sephadex分子筛柱纯化后，酶的纯度达到电泳纯，并通过正相硅胶柱和反相C-18柱分离纯化其反应产物。

Objective To study the conditions and effects of purification of the extract of Ligusticum chuanxiong Hort. by adopting macroporos resin.

目的 探讨大孔吸附树脂对川芎提取物的纯化条件及纯化效果。

METHOUS: An aqueous extract of Rhizoma menispermi, an alcohol purified extract named phenolic alkaloids of menispermum dauricum and Rhizoma menispermi polysaccharide were isolated and purified by using the three-step method.

用三步法初步分离、纯化北豆根提取物，得到北豆根水提液(aqueous extract of rhizome menispermi， RMAE)、醇提液的初步纯化产物蝙蝠葛酚性生物碱(phenolic alkaloids of menispermum dauricum， PAMI)和北豆根多糖成分(rhizome menispermi polysaccharide， RMP)。

ABSTRACT OBJECTIVE:To purify the extract from pleurotus ostreatus,and study on the physicochemical properties and antitumor activity in vitro of the purified component.

目的：对糙皮侧耳真菌提取液进行纯化，并对纯化组分的理化性质及体外抗肿瘤作用进行研究。

The first passage cells were initially pured by enzyme digestion, then pured by enzyme digestion and repeated strapping alternatively in the second course of transferation and inoculation, the third period cultured cells showed typical clonali growth and were long fusiform fibroid cells with short growth cycle.

首次传代时应用胰酶消化法进行成纤维细胞的初次纯化，第2次转种时先后应用酶消化法和反复贴壁法再次进行细胞纯化，第3代以后基本可获得呈典型克隆性生长、长梭形纤维样细胞，生长周期短。

Following attachment to the host immunized with purified antigens, midgut antigens, salivary gland antigens and reinfestation by I. sinensis, the midgut of tick revealed rather strikingly pathological changes, especially of the tick feeding on host immunized with 105KD purfied antigens the basal lamina became thinner, looser, and broken.

中华硬蜱叮咬纯化抗原、中肠抗原、唾液腺抗原免疫接种组和再次感染组宿主后，中肠可发生一系列明显的病理变化，尤以叮咬105KD纯化抗原组宿主后中肠病理变化最为严重。

Methods The method of sucrose gradient centrifugation was used to purify Human herpesvirus-6B(HHV-6B); and in order to make sure the range of positive samples collection, the viron was detected by the methods of PCR, Dot blotting, Western blotting.

采用蔗糖密度梯度离心法进行病毒纯化；通过PCR法、斑点杂交法、蛋白免疫印迹法对纯化样品中HHV-6B进行鉴定，以确定收集阳性样品的范围。

Further isomerization and substitution of this central intermediate leads to the synthesis of other flavonoids. CHSs has been isolated from different plant species, including both monocots and dicots, some of their genomic DNAs and/or cDNAs have also been isolated.

目前，包括单子叶和双子叶植物的很多物种中，有的物种查尔酮合成酶已经得到纯化且基因组、cDNA克隆得到分离，有的或者得到了纯化酶，或者分离得到了基因组、cDNA克隆。

METHODS Using heparin sepharose Cl-6B chromatography,the osteoinduction protein in reconstituted bone xenograftwas pu-rified and fractions obtained from purification were bioassayed for bone-induction activity by implantation into the thigh muscles of mouse.

采用肝素亲和层析技术纯化RBX中诱骨活性蛋白即骨形态发生蛋白，利用小鼠肌袋实验检测其骨诱导活性，再用聚丙烯酰胺电泳测量RBX中诱骨活性蛋白纯化后的相对分子质量。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。