纯化的

The distributions of aminoacylase and alkaline phosphatase had been studied in the common seaweeds in Dalian area, and the Ulva pertusa Kjellm had been chosen as the target for isolation and purification. These two enzymes had been purified for a single band in the SDS-PAGE and their basic characteristics had been studied, too.

研究了氨基酰化酶和碱性磷酸酶在大连地区常见海藻中的分布状况，从中筛选出孔石莼作为纯化研究对象，并从孔石莼中分离纯化出了这两种酶，纯度达到了聚丙烯酰胺凝胶电泳一条带。

This second exhibit can exhibit times Warton 8 inches, wait for whole set product 4 inches with family expenses film, ultralow pressure, low pressure, fight pollute and fight the international such as oxidation banner technology and come to a company a few years be in briny desalt, sewage purification, medium water is answered use, drinking water industrial water is rarefied, rarefied and the arrogant person outstanding achievement that condenses the field place such as depart to obtain and applied technology.

本次展会展出了时代沃顿8寸、4寸和家用膜等全系列产品，超低压、低压、抗污染和抗氧化等国际领先技术以及几年来公司在海水淡化、污水净化、中水回用、饮用水纯化、工业水纯化以及浓缩分离等领域所取得的骄人业绩和应用技术成果。

Methods Viruses were passed in embryonated hen eggs, and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit.

方法病毒在鸡胚中传代，从收获的尿囊液中提取病毒粒RNA，通过逆转录合成cDNA.cDNA通过PCR进行扩增，接着PCR产物用纯化试剂盒进行纯化。

To exploit highly bioactive natural antifungal medicaments from starfish, water-soluble starfish glycosides from Asterias rollestoni were isolated and purified by macroporous resin column and silica gel column chromatography, and their antifungal bioactivities were also examined.

为了分离纯化出具有强抗真菌活性的水溶性海星皂苷，以罗氏海盘车腕为实验材料，利用水抽提方法获得水溶性海星皂苷粗品，依次利用大孔树脂和硅胶柱层析对水溶性海星皂苷进行了纯化，并对其抗真菌活性进行了测定。

For purification of McAb,the results suggested that ion exchange chromatography method can be applied for analysis and caprylic acid method for a very large volume of ascitic fluid.

单抗纯化结果显示离子交换层析法适用于科研分析，而辛酸沉淀法则适用于大量腹水中单抗的纯化。

Methods: Biological technique, including centrifugalization, ion-exchange chromatography, hydrophobic chromatography and dialysis was used. Osteopontin from bovine milk were identified by SDS-PAGE and Western Blot.

运用生物化学技术，通过离心脱脂后，再经过离子交换层析、疏水层析、透析等技术达到分离纯化目的；随后对分离纯化得到的产物进行SDS-PAGE电泳、免疫印迹技术鉴定。

Results indicate that the optimum extraction conditions are as follows: ultrasonic power is 600W, temperature is 53℃, the ratio of liquid to solid is 8︰1, ultrasonic treating time is 70min, reflux extraction time is 120min, the extraction rate of oleanolic acid is 3.000 mg/g; XDA-1 resin is favorable in purification of OA in Chaenomeles sinensis Koehne, the optimum purification conditions are found to be 0.794 mg/mL of OA concentration, the flow rate of adsorption is 0.5 mL/min; the desorption solvent is 90% ethanol, and the flow rate of elutant is 1.0 mL/min.

结果表明，超声波辅助提取最佳工艺条件为：超声功率600 W、提取温度53℃、液料比8：1、超声波提取70 min，回流提取120 min，齐墩果酸得率为3.000 mg/g；XDA-1型大孔树脂对齐墩果酸具有较好的吸附和解吸效果，最佳纯化工艺条件为上柱液浓度0.794 mg/mL，吸附流速0.5 mL/min；以90%浓度乙醇洗脱，洗脱流速1.0 mL/min，纯化后得提取物中齐墩果酸纯度可达26.55%。

Methods:Fibrinogen was purified by precipitation from citrated plasma by saturated ammonium sulplvate.Fibrinogen-bound sialic acid levels with 22 controls and 113 liver patients were assayed by the thiobarbituric acid method.

应用饱和硫酸铵制备纯化纤维蛋白原，硫代巴比妥酸法测定22例正常人，133例肝病患者血浆FSA水平，并对血浆和纯化纤维蛋白原脱唾液酸化前后的凝血酶时间和纤维蛋白单体聚合浊度进行测定。

Firstly, MiCy, Citrine, mKO were inser into expression vectors of pRSETB, and then MiCy, Citrine,mKO were expressed in E.coli and purified by using Ni-NTA collum chromatography.

首先，构建了含有六个组氨酸标签的三种荧光蛋白MiCy、Citrine和mKO，而后在大肠杆菌中表达了MiCy、mKO和Citrine三种蛋白，并用金属鳌合亲和层析进一步纯化，纯化产物使用特异性酶去除His-tag。

By a method using fraction precipitation of ammonium sulphate, ion exchange, modified hydrorylapatite and gel filtration chromatography, we separated and purified two kinds of phycoerythrin(R-PE and r-PE) from Corallina officinalis . The purity standard(ratio of ODs65/OD2go) of R-PE reaches to 11, which is the highest purity comparing with the reported purity of phycoerythrin.

经盐析、离子交换层析、羟基磷灰石吸附层析和凝胶过滤层析等分离纯化方法，从珊瑚藻中分离纯化出两种藻红蛋白，其中R-PE纯度OD_(565／OD_(280)高达11左右，是目前国内外报道的藻红蛋白中纯度最高。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。