纯化的

After induction and massive expression of the 3 fusion proteins, the soluble fraction was purified with 50% Ni-NTA resin affinity chromatography, which was proven by SDS-PAGE of the eluate to be a method that can achieve high purity of fusion proteins.

大量诱导表达三种融合蛋白后，取含FA、FB融合蛋白的可溶性组分用50％Ni-NTA树脂过柱纯化，将洗涤部分进行SDS-PAGE检测，表明用此纯化方法能纯化到高纯度的融合蛋白。

Large and medium scale air separation unit ; Molecular sieve purification system ; Adsorbent ; Uniform distribution ; Energy save

阐述了大中型空分设备分子筛纯化系统的技术新进展，详细分析了分子筛纯化系统设计以及运行中常见故障的预防和处理，对分子筛纯化系统设计制造方面的进步进行了展望。

By n-butanol extraction, ammonium sulfate fractionation and sodium chloride dialyzation, the partially purified enzyme was gained by means of gel filtration on sephadex G-150 column, and some properties of the enzyme were determined.

方法］以DFRKN-1为提酶材料，经正丁醇提取，硫酸按分级沉淀分离，透析获得酶的粗提取液，用SephadexG-150凝胶过滤柱层析纯化，获得纯化碱性磷酸酶，并测定纯化碱性磷酸酶的理化性质。

Five mice of each group were challenged with 100 LD50 of Erysipelothrix rhusiopathiae virulent strain C43065 two weeks after the third immunization, and the specific antibody responses for SpaA was determined by indirect ELISA.

SDS-PAGE结果显示，采用GST Bind Resin纯化试剂盒和电洗脱法纯化得到了66 kDa的rSpaA-N和64 kDa的天然SpaA，蛋白含量分别为1.34 mg/mL 和1.26 mg/mL，而Western印迹结果表明rSpaA-N和纯化前后的SpaA具有良好的免疫反应性。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

The analysis of scanning electromicroscope showed that it didn't obviously exist protein granules in rice starch pured by alcalase, and the solubility and swelling power of rice starch increased respectively after purification.

扫描电镜分析大米淀粉的超微结构显示，碱性蛋白酶纯化处理后的大米淀粉中未见明显的蛋白质颗粒存在。比较纯化前后的大米淀粉发现，大米淀粉经过碱性蛋白酶纯化后，其溶解度和膨润力都明显增加。

The analysis results of scanning electromicroscope showed that protein granules didn't obviously exist in rice starch pured by alcalase, and the solubility and swelling power of rice starch increased remarkably after purification.

扫描电镜分析大米淀粉的超微结构显示，碱性蛋白酶纯化处理后的大米淀粉中未见明显的蛋白质颗粒存在。比较纯化前后的大米淀粉发现，大米淀粉经过碱性蛋白酶纯化后，其溶解度和膨润力都明显增加。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

Dorsalis by salting out, ion exchange chromatography, and gel filtration chromatography. By salting out the egg homogenate, SDS-PAGE analysis revealed that the Vns were mainly precipitated in a 50~70% ammonium sulfate solution. The salted-out proteins were further purified by ion exchange chromatography, and the result showed that Vns were mainly eluted with a 0.3~0.5 M sodium chloride gradient. Finally, the elutes of ion exchange chromatography were refined by running them through gel filtration chromatography to obtain purified Vns. SDS-PAGE separated the 48- and 51-kDa purified Vns, and used them as antigens to raise polyclonal antibodies.

利用硫酸铵盐析法初步分离果实蝇卵中的蛋白质，以SDS-PAGE蛋白质电泳分析得知，卵黄蛋白主要在硫酸铵溶液浓度为50~70%时被沉淀出；盐析沉淀所得之卵黄蛋白，再以离子交换层析法进一步纯化显示，卵黄蛋白主要会在0.3~0.5 M氯化钠的浓度范围间被析出来；再将析出液浓缩后，经凝胶过滤层析法做最后的纯化，即获得纯化之卵黄蛋白。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管，4～6h增菌后，加入适量甘油-生理盐水保存液，-20℃冰箱保存；链球菌属和奈瑟氏菌属的菌株纯化后，用接种环取菌置于新鲜脱纤维绵羊血中，加入适量保存液，-80℃低温冰箱保存；真菌菌种纯化后接种于带硅胶塞的半固体培养基，25℃培养24h后，-20℃冰箱保存。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。