纯化的

It was considered an effective and ideal model of PM.2 uPA,PAI-1 and TNF-αwere all involved in the pathogenesis of PM and participated in the process of the degeneration,apoptosis and necrosis of the diseased muscle.3 MMP-9 and TIMP-2 immunoreactivity were positive in the capillary,venous wall,arteriola endotheliocyte and neurilemma in the normal group skeletal muscle,but more intensive in EAM.They may aggravate the pathological changes of the muscle in human PM.

结论1兔纯化的肌球蛋白加含卡介苗的完全弗氏佐剂可仿制出人类多发性肌炎的理想动物模型。2 UPA、PAI-1和TNF-α参与多发性肌炎的肌纤维坏变过程。3 MMP-9和TIMP-2在正常肌肉组织中的毛细血管、静脉管壁、动脉肉皮细胞和神经鞘膜表达，在多发性肌炎中，参与加重肌纤维的坏变过程。

The infection rate was significantly decreased if we upregulate Attacin expression by E.coli injection prior to trypanosome feeding.Attacin gene was cloned and expressed in Drosophilla S2 cells. Purified protein shows strong activity against E.coli, as well as trypanosome. But tsetse symbiont Sodalis, a gram negative bacterium, was quite resistant to Attacin.

克隆Attacin基因并在果蝇S2细胞中表达，对纯化的Attacin蛋白的研究表明它除了对大肠杆菌有很强的活性外，对锥虫也有较强的活性，而同为格兰氏阴性菌的采采蝇共生菌Sodalis则在试验中表现了较强的抗性。

The animal model is established successfully. The purified main allergen could greatly improve the test of sIgE titer, which suggest that the purified allergen can be used in hypersensitivity diagnosis and therapy of sea shrimp.

结果表明，海虾过敏症豚鼠模型建立成功，并且纯化的海虾过敏原组分应用于ELISA检测能提高sIgE的检测水平，对海虾过敏症的体外诊断和治疗有一定的意义。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS摘要: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

方法摘要：运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

An enzyme linked immunosorbent assay was developed using heat stress protein 70 purified with its cDNA and pET vector system and the titer of antibody to HSP70 in plasma was determined in 40 control parachutists without parachuting for half year and 42 parachutists after parachuting.

目的：探讨HSP70抗体滴度在评价跳伞对空降兵健康效应中的影响。方法：利用cDNA和pET载体系统纯化的HSP70，建立检测HSP70抗体滴度的酶联免疫吸附鉴定方法，并调查了40名近半年来未跳伞的对照组空降兵和42名跳伞后的跳伞组空降兵血浆中HSP70抗体滴度。

The results showed that the purified recombinant protein under denaturing conditions was refolded in Ni-NTA column by using refolding buffer.

实验结果表明，在变性条件下纯化的样品在柱上不经洗脱，直接用复性液洗涤6h，可以使EKLC在得到提纯的同时实现复性，得到了电泳纯度为96％的具有生物活性的EKLC，最高活性为712U。

Deoxyribonucleic acid is an important biomacromolecule. It is a carrier of genetic information and a critical target for radiobiological effects.

选用HI 13串列加速器产生的不同传能线密度的7Li和12 C重离子，以不同的剂量对纯化的质粒DNA水溶液进行了辐照。

The research of SVMP is mostly related the mechanism of coagulation. Take the SVMP of Naja mocambique mocambique as an example, it will cleave GPIb of platelet, making the bonding of GPIb and VWF factor of membrane failed, then the coagulation will be stopped.

蛇毒金属蛋白酵素的研究大部分与凝血机制有关，例如所要纯化的莫三比克眼镜蛇蛇毒金属蛋白酵素，其会切断血小板上的GPIb，而造成GPIb无法与膜上的VWF factor形成键结，而无法进行凝血的步骤。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。