纯化的

The part one of this thesis was concerned with expression and purification of recombinant low molecular weight sweet potato PAP and generation of a polyclonal antiserum from it.

本论文的第一部分致力于对低分子量的红薯紫色酸性磷酸酶的表达和纯化的研究，并产生相应的抗血清。

The notion that a functioning state must rely upon pure ethnicity and a set of cultural and religious beliefs widely and deeply shared by those within its boundaries may be one of the most destructive ideals of the last millennium.

认为一个有效运行的国家必须依赖纯化的种族以及一整套被国民所普遍接受的宗教与文化信仰，这种观点或许是上一个千年中最具有破坏性的想法之一。

The new model targeted on Cathepsin K was proved to be feasible and effective in the screening for antiosteoporosis drug.

初筛以抑制木瓜蛋白酶的活性为指标，经筛选从1000株真菌中得到9株具有较强抑制效应的真菌，复筛采用对纯化的组织蛋白酶K的特异性抑制效应为指标。

The purified N protein was used to coat 96-well plate, each step was optimized, such as coating concentration of recombinant nucleocapsid protein, scample dilutim, chromogen and stop solution as well as concentration.

在此基础上，以纯化的重组N蛋白作为包被抗原，对各种条件进行优化（如抗原的包被，作用时间及底物的选择），确定了判定标准，建立了检测PRRS抗体的间接ELISA方法。

To address this issue, we investigated the RNA synthesis in vitro from RNA templates corresponding to the 3\' terminuses of HCV positive- and negative-strand RNA by a purified HCV NS5B.

本论文利用纯化的HCV NS5B蛋白以及HCV正链、负链3'末端RNA，在体外条件下研究NS5B利用两种模板进行RNA合成的情况，以探讨HCV基因组模板选择性的机制。

In this thesis, the solution of Chinese reference materials including lithium, potassium, calcium, sodium, magnesium, was used to evaluate analytical methods applied. Lithium was enriched in different eluents (hydrochloric acid, nitric acid and mixture with ethanol or methanol) by cation exchange resin AG 50W-X8, and the optional experimental conditions were studied.

本文以锂元素标准样品和钾、钠、钙、镁元素标准样品的混合溶液为主要研究对象，采用阳离子交换树脂AG-50W-X8来分离富集锂，探索在不同淋洗介质(包括盐酸、硝酸以及与甲醇、乙醇的混合)条件下锂分离纯化的最佳介质条件。

Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原，免疫BALB/c小鼠，按常规方法进行融合，间接ELISA方法进行筛选，采用有限稀释法，经过三次克隆，最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5)，分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类，三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

The appreciable mitogenic activity of the purified shaFGF was comparable to that of the haFGF standard.

结果：shaFGF的表达量约为25％，纯化的shaFGF的促分裂活性与haFGF标准品相当。

Results The extracted tubulin could assemble microtubules and form complex with tau in vitro.

结果电子显微镜负染显示纯化的微管蛋白在一定的实验条件下可聚集形成直径为25 nm的微管结构。

The purified human recombined TNFαwas solidated, and antibodies against TNFαdisplayed on phage was enriched on it by five rounds of biopanning, including attach-elute-proliferation, from the pooled human antibody combinatorial libraries .

将纯化的人重组TNFα固相化，用混合的噬菌体人源抗体库(HIV、HAV 和HBV)进行五轮&吸附-洗脱-扩增&筛选，使能够与TNFα特异性结合的噬菌体抗体得到富集。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。