纯化的

But it showed intact function to rebind to PSⅡ membrane and recover its oxygen-evolving activity.

纯化的野生型表达蛋白在结构上和天然蛋白有所不同，但却保持了完整的结合PSⅡ膜能力及恢复放氧的功能。

A complex sample containing BMP and sodium fluoride was implanted under the abdominal skin of young male Wistar rats.

在纯化的BMP中加入氟化物再植入大鼠皮下，通过比较植入物在不同植入时间的组织学变化，碱性磷酸酶、酸性磷酸酶活性及钙含量来反映BMP的生物活性。

The method comprises steps as follows:(1) Chinese herbal medicines are taken and added with purified water for soaking, with the material-to-water ratio of 1:10;(2) the Chinese herbal medicines with the purified water are placed in a microwave heating system; the power of the microwave and the radiation extraction time are controlled;(3) leaching liquor is poured out and put into a centrifuge for centrifugation to obtain the Chinese herbal medicine water extract;(4) polyethersulfone ultrafiltration film is arranged in an ultrafilter; the Chinese herbal medicine water extract is poured into the ultrafilter and placed in a normal pressure type microwave heating system; and (5) the power of the microwave and the radiation extraction time are controlled and the pressure of nitrogen is regulated so as to obtain the purified Chinese herbal medicine water extract.

本发明公开了一种利用微波辐射改善中草药水提液膜超滤过程的方法，该方法由以下步骤完成：(1)取中草药材以1∶10的料液比加入纯净水，浸泡；(2)置于微波加热系统，控制微波功率和辐射提取时间；(3)倾出浸提液；然后用离心机离心得中草药水提液；(4)超滤器内置有聚醚砜超滤膜，将中草药水提液倒入超滤器并置于常压式微波加热系统；(5)控制微波功率和辐射提取时间，调节氮气压力，得到纯化的中草药水提液。

It is a real time technology for the monitoring of dynamic progress of reactions, determination of kinetic constants, analyses of label-free and unpurified biological samples with very high sensibility and non-background interference.

它既可实时监测反应的动态过程、测定动力学常数，又能直接分析未经标记和纯化的生物样品，具有很高的灵敏度，且没有背景干扰。

The optimum process was as follows : solvent is 0.3% ammonia solution , ratio of solvent to solid ratio is 10:1 , vacuum-cavitated time is 15 minutes , GA extraction yeild is 2.0%, the crude extract is 30.3%, and in which the content of GA is 6.6%.

采用负压空化方法对超临界萃取后固形物中甘草酸的提取工艺进行了研究，并研究了甘草酸纯化的工艺，通过影响因素试验和正交试验得出最佳工艺条件为：0.3%的稀氨水为溶剂，液固比为10：1，负压空化提取15min，甘草酸提取率为2.0%，含量6.6%。

The method takes microcrystalline cellulose as affinity column chromatographic medium of Beta-glucosaccharase; under low temperature and anacid condition, the sample of Beta-glucosaccharase is loaded and specifically adsorbed to the microcrystalline column, and then the concentration of NaCl is increased to elute the enzyme, so as to achieve high-efficient and economic separation and purification.

本方法以微晶纤维素作为β-葡萄糖苷酶的亲和柱层析介质，在低温和微酸性条件下，将β-葡萄糖苷酶上样并特异性吸附于微晶纤维素柱，然后再通过增加NaCl浓度的方法将该酶洗脱，从而达到其高效而经济分离纯化的目的。

Results The in vitro coupled bacterial luciferase:FMN-NADH oxidoreductase bioluminescent system was: 1mL crude extract, 27 mmol/L Dodecane 100 μL, 10 mmol/L FMN-Na 0.5 μL and 0.14 mmol/L NADH 300 μL.

利用从鳆发光杆菌提取并经部分纯化的荧光素酶和FMN-NADH氧化还原酶，通过优化体系中各底物的添加量，实现荧光素酶的体外发光。

All IBDV BC 6/85 F4 and IBDV HE BEI isolated strain were obtained from allantochonon and allantoic fluid of died embryonated eggs in 2-5 days after inoculating and virus-infected cell fluid that were frozen and thawed three times after approximately 80% of the monolayer exhibited cytopathic effects.

将纯化的IBDV分别免疫家兔和小鼠，制备了兔抗IBDV、鼠抗IBDV的高免血清，用健康兔的血清免疫羊而获得羊抗兔免疫血清。

Western Blotting showed the gene was expressed specificly in the late stage of erythrocytic stage.

在恶性疟原虫体外抑制实验中，纯化的抗Pfpcp IgG不能有效的抑制两种虫株的生长。

The full-length of cDNA in positive library plasmid pGADal was 1298bp. It encoded a polypeptide of 376 amino acids, which was just the same as the actin gene of Heliothis armigera.

为了确证HaNPV VP39与Actin的相互作用，将纯化的Actin蛋白和HaNPVVP39蛋白通过Western blot和等温量热滴定仪分析两者在体外的相互作用。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。