纯化的

A complex sample containing BMP and sodium fluoride was implanted under the abdominal skin of young male Wistar rats. The histiological characteristcs, and activities of alkine phosphatase and acid phosphatase of the implantan were examined to study the effects of fluoride on biological activities of BMP.

在纯化的BMP中加入氟化物再植入大鼠皮下，通过比较植入物在不同植入时间的组织学变化，碱性磷酸酶、酸性磷酸酶活性及钙含量来反映BMP的生物活性。

A water soluble polysaccharide was isolated and purified from the dry leaves of bamboo that grows in Hubei Province.

采用纸色谱和气相色谱法研究了从湖北恩施地区生长的箬竹叶中分离纯化的水溶性多糖的单糖组成。

The partially purified protease removed the blood stain more effectively when combined with different detergents than the detergents alone.

部分纯化的蛋白酶结合不同洗涤剂比单独的洗涤剂更有效的去除血渍。

Coli were purified and used as the coating antigen in indirect ELISA test, and the results were compared with those obtained by the traditional tube agglutination test method in 100 serum samples in general survey for the Brucella infection.

以大肠杆菌表达、纯化的BP26和OMP31重组蛋白为包被抗原，初步建立了检测血清特异抗体的间接ELISA方法，并以现地牛布氏杆菌普查检测血清100份为样本，与传统的试管凝集试验进行了比较研究。

Conotoxins were isolated, including 23, 25 and 5 peptides from conus textile, conus imperialis and conus marmoreus, respectively.

对分离纯化的芋螺毒素进行了HPLC分析，证实了获得的芋螺毒素的纯度均达到85%以上。

Identify the structure of the unknown amino acid from the melting point and equivalent weight of the purified amino acid derivative.

利用已纯化的胺基酸的衍生物的等当量和熔点，可以对未知胺基酸进行结构鉴定

This technique was modified as follows: the small rubber particles were purified and diluted to give a protein concentration of an absorbance at 600 nm of about 2.0～2.5; 0.5% basic fuchsin was prepared by being dissolved in 50 mmol/L Tris-HCl(pH7.4); the reaction mixtures were incubated for 30 min at 25 ℃, and centrifuged for 5 min at a speed of 5 000 r/min; and B-serum can serve as the protein samples for the positive control of rubber particle aggregation.

几个关键参数为：（1）使用纯化的小橡胶粒子，适宜浓度为在600 nm处的OD值为2.0～2.5；（2）用50 mmol/L Tris-HCl（pH7.4）配制0.5％碱性品红；（3）25 ℃条件下孵育30 min；（4）5 000 r/min离心5 min；（5）B乳清可作为阳性对照的蛋白质样品。

The thymidine kinase gene of infectious laryngotracheitis virus Beijing E2 strain was amplified by PCR, cloned into pGEM-T vector, and sequenced. Sequence analyses showed that the TK gene was very closed among sequenced ILTV strains.

以纯化的传染性喉气管炎病毒北京E2株基因组DNA为模板，通过PCR方法扩增了ILTV北京E2株的完整TK基因，将其克隆到pGEM-T载体上，并测定了序列，与其它ILTV毒株TK基因比较说明，ILTV不同毒株的TK基因高度保守。

There is a typical peak determined by cappillary zone electrophoresis, with a peak area of 100%. It was shown that the isolated FasIg fusion protein be much purificatory.

最后，采用毛细管区带电泳鉴定呈现典型的一个峰，峰面积达100％，表明所分离纯化的Fas-Ig融合蛋白具有很高的纯度。

The method used to separate and purify the enzyme in this experiment was feasible.

本试验选用的分离纯化的方法是可行的。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失